Monoamine Oxidase B Recombinant Rabbit Monoclonal Antibody [JG39-97]
cat.: ET7108-98
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JG39-97 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 59 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Monoamine Oxidase B aa 1-126 / 520. |
| Positive control: | Human liver tissue lysate, SiHa cell lysate, human liver tissue, human prostate cancer tissue, human kidney tissue. |
| Subcellular location: | Mitochondrion. |
| Recommended Dilutions:
WB IHC-P |
1:500 1:50-1:500 |
| Uniprot #: | SwissProt: P27338 Human |
| Alternative names: | Adrenalin oxidase Amine oxidase (flavin containing) Amine oxidase [flavin-containing] B AOFB_HUMAN MAO, brain MAO, platelet MAO-B MAOB MGC26382 Monoamine oxidase B Monoamine oxidase type B OTTHUMP00000023166 Tyramine oxidase |
Images
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Fig1:
Western blot analysis of Monoamine Oxidase B on different lysates with Rabbit anti-Monoamine Oxidase B antibody (ET7108-98) at 1/500 dilution. Lane 1: Human liver tissue lysate (20 µg/Lane) Lane 2: SiHa cell lysate (10 µg/Lane) Predicted band size: 59 kDa Observed band size: 59 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-98) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig2: Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-Monoamine Oxidase B antibody. Counter stained with hematoxylin. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Monoamine Oxidase B antibody (ET7108-98) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-98) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Monoamine Oxidase B antibody (ET7108-98) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-98) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.