Retinoid X Receptor alpha Recombinant Rabbit Monoclonal Antibody [JG99-38]
cat.: ET7108-99
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat, Mouse |
| Applications: | WB, IF-Cell, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | JG99-38 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 51 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein corresponding to Human Retinoid X Receptor alpha aa 1-50 / 462. |
| Positive control: | A549 cell lysate, MCF-7 cell lysate, A549, Hela, rat liver tissue, human esophagus tissue, human skin tissue. |
| Subcellular location: | Mitochondrion, Nucleus, Cytoplasm. |
| Recommended Dilutions:
WB IHC-P IF-Cell IP |
1:500-1:2,000 1:50-1:400 1:50 Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P19793 Human | P28700 Mouse | Q05343 Rat |
| Alternative names: | FLJ00280 FLJ00318 FLJ16020 FLJ16733 MGC102720 NR2B1 Nuclear receptor subfamily 2 group B member 1 OTTHUMP00000022510 Retinoic acid receptor RXR alpha Retinoic acid receptor RXR-alpha Retinoid X nuclear receptor alpha Retinoid X receptor alpha RXR alpha1 Rxra RXRA_HUMAN RXRalpha1 |
Images
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Fig1:
Western blot analysis of Retinoid X Receptor alpha on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7108-99, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: A549 cell lysate Lane 2: MCF-7 cell lysate |
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Fig2: ICC staining of Retinoid X Receptor alpha in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7108-99, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig3: ICC staining of Retinoid X Receptor alpha in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7108-99, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig4: Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-Retinoid X Receptor alpha antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-99, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human esophagus tissue using anti-Retinoid X Receptor alpha antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-99, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-Retinoid X Receptor alpha antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-99, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig7:
Western blot analysis of Retinoid X Receptor alpha on different lysates with Rabbit anti-Retinoid X Receptor alpha antibody (ET7108-99) at 1/500 dilution. Lane 1: A549 cell lysate Lane 2: PC-12 cell lysate Lane 3: Human liver tissue lysate Lane 4: Mouse liver tissue lysate Lane 5: Mouse heart tissue lysate Lane 6: Rat liver tissue lysate Lane 7: Rat heart tissue lysate Cell lysates/proteins at 20 µg/Lane. Tissue lysates/proteins at 40 µg/Lane. Predicted band size: 51 kDa Observed band size: 55 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-99) at 1/500 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.