MGEA5 Recombinant Rabbit Monoclonal Antibody [JG40-05]
cat.: ET7109-01
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | JG40-05 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 103 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human MGEA5 aa 2-103 / 916. |
| Positive control: | PC-3M cell lysate, SH-SY5Y cell lysate, human tonsil tissue, human pancreas tissue, mouse testis tissue. |
| Subcellular location: | Cytoplasm. Nucleus. |
| Recommended Dilutions:
WB IHC-P IP |
1:500-1:2,000 1:50-1:200 1:10-1:50 |
| Uniprot #: | SwissProt: O60502 Human | Q9EQQ9 Mouse |
| Alternative names: | Beta-hexosaminidase Beta-N-acetylglucosaminidase Beta-N-acetylhexosaminidase Bifunctional protein NCOAT HEXC Hyaluronidase in meningioma KIAA0679 MEA5 Meningioma expressed antigen 5 Meningioma-expressed antigen 5 MGEA 5 Mgea5 N-acetyl-beta-D-glucosaminidase N-acetyl-beta-glucosaminidase NCOAT Nuclear cytoplasmic O GlcNAcase and acetyltransferase Nuclear cytoplasmic O-GlcNAcase and acetyltransferase OGA OGA_HUMAN Protein O-GlcNAcase |
Images
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Fig1:
Western blot analysis of MGEA5 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7109-01, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: PC-3M cell lysate Lane 2: SH-SY5Y cell lysate |
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Fig2:
Western blot analysis of MGEA5 on different lysates with Rabbit anti-MGEA5 antibody (ET7109-01) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: JAR cell lysate Lane 3: HEK-293 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 103 kDa Observed band size: 130 kDa Exposure time: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7109-01) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-MGEA5 antibody (ET7109-01) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-01) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-MGEA5 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-01, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-MGEA5 antibody (ET7109-01) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-01) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded rat colon tissue with Rabbit anti-MGEA5 antibody (ET7109-01) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-01) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig7:
MGEA5 was immunoprecipitated from 0.2 mg HeLa cell lysate with ET7109-01 at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using ET7109-01 at 1/5,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: ET7109-01 IP in HeLa cell lysate Lane 3: Rabbit IgG instead of ET7109-01 in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 30 seconds; ECL: K1801 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.