VAMP1 Recombinant Rabbit Monoclonal Antibody [JG40-19]
cat.: ET7109-03
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Cell, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | JG40-19 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 13 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant full length protein of human VAMP1. |
| Positive control: | Mouse cerebellum tissue lysate, rat brain tissue lysate, 293T, Hela, SH-SY-5Y, human prostate cancer tissue, mouse brain tissue. |
| Subcellular location: | Mitochondrion. Synapse. Synaptosome. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue IHC-P |
1:500-1:2,000 1:50-1:200 1:50-1:200 1:50-1:200 |
| Uniprot #: | SwissProt: P23763 Human | Q62442 Mouse | Q63666 Rat |
| Alternative names: | DKFZp686H12131 SYB 1 SYB1 Synaptobrevin 1 Synaptobrevin-1 Synaptobrevin1 VAMP 1 VAMP-1 Vamp1 VAMP1_HUMAN Vesicle associated membrane protein 1 Vesicle associated membrane protein 1 synaptobrevin 1 Vesicle-associated membrane protein 1 |
Images
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Fig1:
Western blot analysis of VAMP1 on different lysates with Rabbit anti-VAMP1 antibody (ET7109-03) at 1/500 dilution. Lane 1: Mouse cerebellum tissue lysate Lane 2: Rat brain tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 13 kDa Observed band size: 15 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7109-03) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig2: ICC staining VAMP1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining VAMP1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining VAMP1 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-VAMP1 antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-VAMP1 antibody. Counter stained with hematoxylin. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.