RACK1 Recombinant Rabbit Monoclonal Antibody [JG40-26]
cat.: ET7109-04
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, FC
Clonality: Monoclonal
Clone number: JG40-26
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 35 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human RACK1 aa 190-317 / 317.
Positive control: SH-SY5Y cell lysate, Daudi cell lysate, rat cerebellum tissue, rat liver tissue, human colon tissue, mouse skin tissue, SH-SY5Y.
Subcellular location: Cytoplasm. Cell membrane. Nucleus.
Recommended Dilutions:
  WB
  IHC-P
  FC
1:1,000-1:2,000
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: P63244 Human | P63245 Rat
Alternative names: Cell proliferation-inducing gene 21 protein GBLP_HUMAN Gnb2-rs1 Gnb2l1 Guanine nucleotide binding protein (G protein) beta polypeptide 2 like 1 Guanine nucleotide binding protein beta polypeptide 2 like 1 Guanine nucleotide binding protein beta subunit 2 like 1 Guanine nucleotide binding protein beta subunit like protein 12.3 Guanine nucleotide binding protein subunit beta 2 like 1 Guanine nucleotide binding protein subunit beta like protein 12.3 Guanine nucleotide-binding protein subunit beta-2-like 1 Guanine nucleotide-binding protein subunit beta-like protein 12.3 H12.3 HLC-7 Human lung cancer oncogene 7 protein lung cancer oncogene 7 OTTHUMP00000223704 OTTHUMP00000223870 OTTHUMP00000223891 OTTHUMP00000223893 OTTHUMP00000223900 OTTHUMP00000223902 OTTHUMP00000223930 OTTHUMP00000223931 PIG21 Proliferation inducing gene 21 Protein homologous to chicken B complex protein guanine nucleotide binding RACK1 Receptor for activated C kin......
Images
ET7109-04_1.jpg Fig1: Western blot analysis of RACK1 on different lysates with Rabbit anti-RACK1 antibody (ET7109-04) at 1/1,000 dilution.

Lane 1: SH-SY5Y cell lysate
Lane 2: Daudi cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 35 kDa
Observed band size: 35 kDa

Exposure time: 43 seconds;

12% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7109-04) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
ET7109-04_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue using anti-RACK1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-04, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7109-04_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-RACK1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-04, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7109-04_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-RACK1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-04, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7109-04_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-RACK1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-04, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7109-04_6.jpg Fig6: Flow cytometric analysis of RACK1 was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7109-04, 1/50) (purple). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; yellow).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.