RACK1 Recombinant Rabbit Monoclonal Antibody [JG40-26]
cat.: ET7109-04
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JG40-26 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 35 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human RACK1 aa 190-317 / 317. |
| Positive control: | Daudi cell lysate, C2C12 cell lysate, C6 cell lysate, Mouse liver tissue lysate, Rat liver tissue lysate, SH-SY5Y cell lysate, human liver tissue, mouse liver tissue, rat liver tissue, SH-SY5Y. |
| Subcellular location: | Cytoplasm. Cell membrane. Nucleus. |
| Recommended Dilutions:
WB IHC-P FC |
1:1,000-1:2,000 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: P63244 Human | P63245 Rat |
| Alternative names: | Cell proliferation-inducing gene 21 protein GBLP_HUMAN Gnb2-rs1 Gnb2l1 Guanine nucleotide binding protein (G protein) beta polypeptide 2 like 1 Guanine nucleotide binding protein beta polypeptide 2 like 1 Guanine nucleotide binding protein beta subunit 2 like 1 Guanine nucleotide binding protein beta subunit like protein 12.3 Guanine nucleotide binding protein subunit beta 2 like 1 Guanine nucleotide binding protein subunit beta like protein 12.3 Guanine nucleotide-binding protein subunit beta-2-like 1 Guanine nucleotide-binding protein subunit beta-like protein 12.3 H12.3 HLC-7 Human lung cancer oncogene 7 protein lung cancer oncogene 7 OTTHUMP00000223704 OTTHUMP00000223870 OTTHUMP00000223891 OTTHUMP00000223893 OTTHUMP00000223900 OTTHUMP00000223902 OTTHUMP00000223930 OTTHUMP00000223931 PIG21 Proliferation inducing gene 21 Protein homologous to chicken B complex protein guanine nucleotide binding RACK1 Receptor for activated C kin...... |
Images
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Fig1:
Western blot analysis of RACK1 on different lysates with Rabbit anti-RACK1 antibody (ET7109-04) at 1/1,000 dilution. Lane 1: Daudi cell lysate (20 µg/Lane) Lane 2: C2C12 cell lysate (20 µg/Lane) Lane 3: C6 cell lysate (20 µg/Lane) Lane 4: Mouse liver tissue lysate (40 µg/Lane) Lane 5: Rat liver tissue lysate (40 µg/Lane) Predicted band size: 35 kDa Observed band size: 32 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7109-04) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of RACK1 on different lysates with Rabbit anti-RACK1 antibody (ET7109-04) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: Daudi cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 35 kDa Observed band size: 35 kDa Exposure time: 43 seconds; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7109-04) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-RACK1 antibody (ET7109-04) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-04) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-RACK1 antibody (ET7109-04) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-04) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-RACK1 antibody (ET7109-04) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-04) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6: Flow cytometric analysis of RACK1 was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7109-04, 1/50) (purple). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; yellow). |
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