Ndufs4 Recombinant Rabbit Monoclonal Antibody [JE40-47]
cat.: ET7109-09
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, FC, IP |
| Clonality: | Monoclonal |
| Clone number: | JE40-47 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 20 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Ndufs4 aa 1-140 / 175. |
| Positive control: | HCT 116 cell lysate, rat heart tissue lysates, human kidney tissue, mouse kidney tissue, rat kidney tissue, SH-SY5Y. |
| Subcellular location: | Mitochondrion. |
| Recommended Dilutions:
WB IHC-P FC IP |
1:500-1:2,000 1:1,000 1:50-1:100 1:10-1:50 |
| Uniprot #: | SwissProt: O43181 Human | Q9CXZ1 Mouse | Q5XIF3 Rat |
| Alternative names: | AQDQ CI 18 CI 18 kDa CI AQDQ CI-18 kDa CI-AQDQ Complex I 18 kDa Complex I AQDQ Complex I-18 kDa Complex I-AQDQ mitochondrial mitochondrial respiratory chain complex I (18 KD subunit) NADH coenzyme Q reductase NADH dehydrogenase (ubiquinone) Fe S protein 4 18kDa NADH dehydrogenase [ubiquinone] iron-sulfur protein 4 NADH dehydrogenase NADH ubiquinone oxidoreductase 18 kDa subunit NADH-ubiquinone oxidoreductase 18 kDa subunit NDUFS4 NDUS4_HUMAN |
Images
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Fig1:
Western blot analysis of Ndufs4 on different lysates with Rabbit anti-Ndufs4 antibody (ET7109-09) at 1/2,000 dilution. Lane 1: HCT 116-si NT cell lysate Lane 2: HCT 116-si Ndufs4 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 20 kDa Observed band size: 20 kDa Exposure time: 1 minute; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7109-09) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2: Western blot analysis of Ndufs4 on rat heart tissue lysate using anti-Ndufs4 antibody at 1/1,000 dilution. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Ndufs4 antibody (ET7109-09) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-09) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-Ndufs4 antibody (ET7109-09) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-09) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-Ndufs4 antibody (ET7109-09) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-09) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6: Flow cytometric analysis of SH-SY5Y cells with Ndufs4 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.