Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human, Mouse, Rat |
Applications: | WB, IHC-P, FC, IP |
Clonality: | Monoclonal |
Clone number: | JE40-47 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | 20 kDa |
Isotype: | IgG |
Immunogen: | Recombinant protein within Human Ndufs4 aa 1-140 / 175. |
Positive control: | Rat heart tissue lysate, rat kidney tissue, human small intestine tissue, SH-SY-5Y. |
Subcellular location: | Mitochondrion. |
Recommended Dilutions:
WB IHC-P FC IP |
1:500-1:2,000 1:50-1:200 1:50-1:100 1:10-1:50 |
Uniprot #: | SwissProt: O43181 Human | Q5XIF3 Rat |
Alternative names: | AQDQ CI 18 CI 18 kDa CI AQDQ CI-18 kDa CI-AQDQ Complex I 18 kDa Complex I AQDQ Complex I-18 kDa Complex I-AQDQ mitochondrial mitochondrial respiratory chain complex I (18 KD subunit) NADH coenzyme Q reductase NADH dehydrogenase (ubiquinone) Fe S protein 4 18kDa NADH dehydrogenase [ubiquinone] iron-sulfur protein 4 NADH dehydrogenase NADH ubiquinone oxidoreductase 18 kDa subunit NADH-ubiquinone oxidoreductase 18 kDa subunit NDUFS4 NDUS4_HUMAN |
Fig1: Western blot analysis of Ndufs4 on rat heart tissue lysate using anti-Ndufs4 antibody at 1/1,000 dilution. | |
Fig2: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-Ndufs4 antibody. Counter stained with hematoxylin. | |
Fig3: Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-Ndufs4 antibody. Counter stained with hematoxylin. | |
Fig4: Flow cytometric analysis of SH-SY-5Y cells with Ndufs4 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |