Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
---|---|
Species reactivity: | Human |
Applications: | WB, IF-Cell, IF-Tissue, IHC-P |
Clonality: | Monoclonal |
Clone number: | JE40-59 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | 141 kDa |
Isotype: | IgG |
Immunogen: | Synthetic peptide within Human DIAPH1 aa 1-50 / 1,272. |
Positive control: | SiHa, LOVO, human liver cancer tissue, human colon tissue. |
Subcellular location: | Cytoskeleton. Plasma membrane. |
Recommended Dilutions:
WB IF-Cell IHC-P |
1:5,000-1:10,000 1:50 1:50-1:100 |
Uniprot #: | SwissProt: O60610 Human |
Alternative names: | DIAPH1 deafness, autosomal dominant 1 DFNA1 DIA1 DIAP1 DIAP1_HUMAN DIAPH1 Diaphanous homolog 1 (Drosophila) diaphanous homolog 1 Diaphanous related formin 1 Diaphanous-related formin-1 DRF1 FLJ25265 hDIA1 LFHL1 low frequency hearing loss 1 p140DIA Protein diaphanous homolog 1 |
Fig1: Western blot analysis of DIAPH1 on SiHa cell lysate using anti-DIAPH1 antibody at 1/5,000 dilution. | |
Fig2: ICC staining DIAPH1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. | |
Fig3: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-DIAPH1 antibody. Counter stained with hematoxylin. | |
Fig4: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-DIAPH1 antibody. Counter stained with hematoxylin. |