DIAPH1 Recombinant Rabbit Monoclonal Antibody [JE40-59]
cat.: ET7109-11
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, IF-Cell, IF-Tissue, IHC-P
Clonality: Monoclonal
Clone number: JE40-59
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: 141 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human DIAPH1 aa 1-50 / 1,272.
Positive control: SiHa, LOVO, human liver cancer tissue, human colon tissue.
Subcellular location: Cytoskeleton. Plasma membrane.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P

1:5,000-1:10,000
1:50
1:50-1:100
Uniprot #: SwissProt: O60610 Human
Alternative names: DIAPH1 deafness, autosomal dominant 1 DFNA1 DIA1 DIAP1 DIAP1_HUMAN DIAPH1 Diaphanous homolog 1 (Drosophila) diaphanous homolog 1 Diaphanous related formin 1 Diaphanous-related formin-1 DRF1 FLJ25265 hDIA1 LFHL1 low frequency hearing loss 1 p140DIA Protein diaphanous homolog 1
Images
ET7109-11_1.jpg Fig1: Western blot analysis of DIAPH1 on SiHa cell lysate using anti-DIAPH1 antibody at 1/5,000 dilution.
ET7109-11_2.jpg Fig2: ICC staining DIAPH1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET7109-11_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-DIAPH1 antibody. Counter stained with hematoxylin.
ET7109-11_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-DIAPH1 antibody. Counter stained with hematoxylin.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.