DIAPH1 Recombinant Rabbit Monoclonal Antibody [JE40-59]
cat.: ET7109-11
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IF-Tissue, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE40-59 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 141 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human DIAPH1 aa 1-50 / 1,272. |
| Positive control: | SiHa, LOVO, human liver cancer tissue, human colon tissue. |
| Subcellular location: | Cytoskeleton. Plasma membrane. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:5,000-1:10,000 1:50 1:50-1:100 |
| Uniprot #: | SwissProt: O60610 Human |
| Alternative names: | DIAPH1 deafness, autosomal dominant 1 DFNA1 DIA1 DIAP1 DIAP1_HUMAN DIAPH1 Diaphanous homolog 1 (Drosophila) diaphanous homolog 1 Diaphanous related formin 1 Diaphanous-related formin-1 DRF1 FLJ25265 hDIA1 LFHL1 low frequency hearing loss 1 p140DIA Protein diaphanous homolog 1 |
Images
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Fig1: Western blot analysis of DIAPH1 on SiHa cell lysate using anti-DIAPH1 antibody at 1/5,000 dilution. |
|
Fig2: ICC staining DIAPH1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
|
Fig3: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-DIAPH1 antibody. Counter stained with hematoxylin. |
|
Fig4: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-DIAPH1 antibody. Counter stained with hematoxylin. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.