PGAM1 Recombinant Rabbit Monoclonal Antibody [JE40-67]
cat.: ET7109-13
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, FC
Clonality: Monoclonal
Clone number: JE40-67
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: 29 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human PGAM1 aa 1-103 / 254.
Positive control: A431, A549, rat brain tissue, mouse brain tissue, human liver tissue, Jurkat.
Subcellular location: Cytosol. Extracellular region or secreted. Nucleus.
Recommended Dilutions:
  WB
  IHC-P
  FC

1:500-1:2,000
1:50-1:100
1:50-1:100
Uniprot #: SwissProt: P18669 Human | Q9DBJ1 Mouse | P25113 Rat
Alternative names: BPG dependent PGAM 1 BPG dependent PGAM1 BPG-dependent PGAM 1 OTTHUMP00000059414 PGAM 1 PGAM A PGAM B PGAM-B PGAM1 PGAM1_HUMAN PGAMA PGAMB Phosphoglycerate mutase 1 Phosphoglycerate mutase 1 brain Phosphoglycerate mutase A Phosphoglycerate mutase A nonmuscle form Phosphoglycerate mutase isozyme B
Images
ET7109-13_1.jpg Fig1: Western blot analysis of PGAM1 on different lysates using anti-PGAM1 antibody at 1/2,000 dilution.
Positive control:
Lane 1: A431
Lane 2: A549
Lane 3: Rat brain
Lane 4: Mouse brain
ET7109-13_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-PGAM1 antibody. Counter stained with hematoxylin.
ET7109-13_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-PGAM1 antibody. Counter stained with hematoxylin.
ET7109-13_4.jpg Fig4: Flow cytometric analysis of Jurkat cells with PGAM1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.