PGAM1 Recombinant Rabbit Monoclonal Antibody [JE40-67]
cat.: ET7109-13
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JE40-67 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 29 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human PGAM1 aa 1-103 / 254. |
| Positive control: | A549 cell lysate, Mouse brain tissue lysate, rat brain tissue, human liver tissue. |
| Subcellular location: | Cytosol. Extracellular region or secreted. Nucleus. |
| Recommended Dilutions:
WB IHC-P FC |
1:2,000 1:50-1:100 1:50-1:100 |
| Uniprot #: | SwissProt: P18669 Human | Q9DBJ1 Mouse | P25113 Rat |
| Alternative names: | BPG dependent PGAM 1 BPG dependent PGAM1 BPG-dependent PGAM 1 OTTHUMP00000059414 PGAM 1 PGAM A PGAM B PGAM-B PGAM1 PGAM1_HUMAN PGAMA PGAMB Phosphoglycerate mutase 1 Phosphoglycerate mutase 1 brain Phosphoglycerate mutase A Phosphoglycerate mutase A nonmuscle form Phosphoglycerate mutase isozyme B |
Images
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Fig1:
Western blot analysis of PGAM1 on different lysates with Rabbit anti-PGAM1 antibody (ET7109-13) at 1/2,000 dilution. Lane 1: A549 cell lysate(20 µg/Lane) Lane 2: Mouse brain tissue lysate(40 µg/Lane) Exposure time: 10 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ET7109-13, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 29 kDa Observed band size: 29 kDa |
|
Fig2: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-PGAM1 antibody. Counter stained with hematoxylin. |
|
Fig3: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-PGAM1 antibody. Counter stained with hematoxylin. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.