Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human, Mouse, Rat |
Applications: | WB, IHC-P, FC |
Clonality: | Monoclonal |
Clone number: | JE40-67 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | 29 kDa |
Isotype: | IgG |
Immunogen: | Recombinant protein within Human PGAM1 aa 1-103 / 254. |
Positive control: | A431, A549, rat brain tissue, mouse brain tissue, human liver tissue, Jurkat. |
Subcellular location: | Cytosol. Extracellular region or secreted. Nucleus. |
Recommended Dilutions:
WB IHC-P FC |
1:500-1:2,000 1:50-1:100 1:50-1:100 |
Uniprot #: | SwissProt: P18669 Human | Q9DBJ1 Mouse | P25113 Rat |
Alternative names: | BPG dependent PGAM 1 BPG dependent PGAM1 BPG-dependent PGAM 1 OTTHUMP00000059414 PGAM 1 PGAM A PGAM B PGAM-B PGAM1 PGAM1_HUMAN PGAMA PGAMB Phosphoglycerate mutase 1 Phosphoglycerate mutase 1 brain Phosphoglycerate mutase A Phosphoglycerate mutase A nonmuscle form Phosphoglycerate mutase isozyme B |
Fig1:
Western blot analysis of PGAM1 on different lysates using anti-PGAM1 antibody at 1/2,000 dilution. Positive control: Lane 1: A431 Lane 2: A549 Lane 3: Rat brain Lane 4: Mouse brain |
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Fig2: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-PGAM1 antibody. Counter stained with hematoxylin. | |
Fig3: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-PGAM1 antibody. Counter stained with hematoxylin. | |
Fig4: Flow cytometric analysis of Jurkat cells with PGAM1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |