Aconitase 2 Recombinant Rabbit Monoclonal Antibody [JE40-83]
cat.: ET7109-15
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IF-Tissue, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | JE40-83 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 85 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Aconitase 2 aa 1-190 / 780. |
| Positive control: | SiHa cell lysate, HL-60 cell lysate, SiHa, rat epididymis tissue, human prostate cancer tissue, human kidney tissue, human esophagus tissue, mouse testis tissue. |
| Subcellular location: | Mitochondrion. |
| Recommended Dilutions:
WB IF-Cell IHC-P IP |
1:500-1:2,000 1:50-1:200 1:50-1:200 1:10-1:50 |
| Uniprot #: | SwissProt: Q99798 Human | Q99KI0 Mouse | Q9ER34 Rat |
| Alternative names: | ACO 2 ACO2 ACON_HUMAN aconitase 2 Aconitase 2 mitochondrial Aconitase Aconitase2 Aconitate hydratase Aconitate hydratase mitochondrial ACONM Citrate hydro lyase Citrate hydro-lyase ICRD mitochondrial |
Images
|
Fig1:
Western blot analysis of Aconitase 2 on different lysates with Rabbit anti-Aconitase 2 antibody (ET7109-15) at 1/500 dilution. Lane 1: SiHa cell lysate Lane 2: HL-60 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 85 kDa Observed band size: 85 kDa Exposure time: 1 minute; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7109-15) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
|
Fig2: ICC staining Aconitase 2 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
|
Fig3: Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-Aconitase 2 antibody. Counter stained with hematoxylin. |
|
Fig4: Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-Aconitase 2 antibody. Counter stained with hematoxylin. |
|
Fig5:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Aconitase 2 antibody (ET7109-15) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-15) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig6: Immunohistochemical analysis of paraffin-embedded human esophagus tissue using anti-Aconitase 2 antibody. Counter stained with hematoxylin. |
|
Fig7:
Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-Aconitase 2 antibody (ET7109-15) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-15) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.