ET7109-17

TTC11/FIS1 Recombinant Rabbit Monoclonal Antibody [JE40-90]

cat.: ET7109-17

Product Type:	Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity:	Human, Mouse, Rat
Applications:	WB, IF-Cell, IHC-P, IF-Tissue
Clonality:	Monoclonal
Clone number:	JE40-90

Form:	Liquid
Storage condition:	Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer:	1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration:	1ug/ul
Purification:	Protein A affinity purified.
Molecular weight:	Predicted band size: 17 kDa
Isotype:	IgG
Immunogen:	Recombinant protein within Human TTC11 aa 1-125 / 152.
Positive control:	Mouse spleen tissue lysate, mouse brain tissue lysate, rat spleen tissue lysate, rat brain tissue lysate, MCF-7, SH-SY-5Y, SK-Br-3, human kidney tissue, human small intestine tissue, human uterus tissue, mouse brain tissue, mouse colon tissue, rat brain tissue, rat colon tissue.
Subcellular location:	Peroxisome. Mitochondrion.
Recommended Dilutions: WB IF-Cell IHC-P IF-Tissue	1:500-1:2,000 1:50-1:200 1:1,000 1:200
Uniprot #:	SwissProt: Q9Y3D6 Human \| Q9CQ92 Mouse \| P84817 Rat
Alternative names:	2010003O14Rik CGI 135 CGI135 FIS 1 FIS1 Fis1 homolog FIS1, S. cerevisiae, homolog of FIS1_HUMAN Fission 1 (mitochondrial outer membrane) homolog (S. cerevisiae) Fission 1 (mitochondrial outer membrane) homolog (yeast) Fission 1 (mitochondrial outer membrane) homolog Fission 1 homolog H NH0132A01.6 hFis 1 hFis1 Mitochondrial fission 1 protein mitochondrial fission molecule Tetratricopeptide repeat domain 11 Tetratricopeptide repeat protein 11 TPR repeat protein 11 TTC 11

Images

	Fig1: Western blot analysis of TTC11/FIS1 on different lysates with Rabbit anti-TTC11/FIS1 antibody (ET7109-17) at 1/1,000 dilution. Lane 1: Mouse spleen tissue lysate Lane 2: Mouse brain tissue lysate Lane 3: Rat spleen tissue lysate Lane 4: Rat brain tissue lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 17 kDa Observed band size: 15 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7109-17) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
	Fig2: Western blot analysis of TTC11 on SK-Br-3 cell using anti-TTC11 antibody at 1/2,000 dilution.
	Fig3: ICC staining TTC11 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

	Fig4: ICC staining TTC11 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
	Fig5: ICC staining TTC11 in SK-Br-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
	Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-TTC11/FIS1 antibody (ET7109-17) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET7109-17) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig7: Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-TTC11/FIS1 antibody (ET7109-17) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET7109-17) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Fig8: Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-TTC11/FIS1 antibody (ET7109-17) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET7109-17) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Fig9: Immunohistochemical analysis of paraffin-embedded rat colon tissue with Rabbit anti-TTC11/FIS1 antibody (ET7109-17) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET7109-17) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.