Syntaxin 3 Recombinant Rabbit Monoclonal Antibody [JE40-96]
cat.: ET7109-19
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE40-96 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 33 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Syntaxin 3 aa 92-218 / 289. |
| Positive control: | 293T cell lysate, HepG2 cell lysate, RAW264.7 cell lysate, C6 cell lysate, mouse kidney tissue, mouse retina tissue, rat kidney tissue, rat retina tissue, human prostate cancer tissue, human kidney tissue. |
| Subcellular location: | Apical cell membrane; Nucleus. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:2,000-1:5,000 |
| Uniprot #: | SwissProt: Q13277 Human | Q64704 Mouse | Q08849 Rat |
| Alternative names: | FLJ30906 Stx3 STX3_HUMAN STX3A Syntaxin 3A Syntaxin-3 |
Images
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Fig1:
Western blot analysis of Syntaxin 3 on different lysates with Rabbit anti-Syntaxin 3 antibody (ET7109-19) at 1/1,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-Syntaxin 3 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 33 kDa Observed band size: 35 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7109-19) at 1/1,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of Syntaxin 3 on different lysates with Rabbit anti-Syntaxin 3 antibody (ET7109-19) at 1/5,000 dilution. Lane 1: 293T cell lysate Lane 2: HepG2 cell lysate Lane 3: RAW264.7 cell lysate Lane 4: C6 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 33 kDa Observed band size: 35 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7109-19) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-Syntaxin 3 antibody (ET7109-19) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-19) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded mouse retina tissue with Rabbit anti-Syntaxin 3 antibody (ET7109-19) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-19) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5:
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-Syntaxin 3 antibody (ET7109-19) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-19) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig6:
Immunohistochemical analysis of paraffin-embedded rat retina tissue with Rabbit anti-Syntaxin 3 antibody (ET7109-19) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-19) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig7: Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-Syntaxin 3 antibody. Counter stained with hematoxylin. |
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Fig8: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Syntaxin 3 antibody. Counter stained with hematoxylin. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.