Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human, Mouse, Rat |
Applications: | WB, IHC-P, FC |
Clonality: | Monoclonal |
Clone number: | JE40-97 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | 77/88 kDa |
Isotype: | IgG |
Immunogen: | Recombinant protein within Human MARK2 aa 661-789 / 789. |
Positive control: | MCF-7, SK-Br-3, human appendix tissue. |
Subcellular location: | Cell membrane. Cytoplasm. Cytoskeleton. |
Recommended Dilutions:
WB IHC-P FC |
1:500-1:2,000 1:50-1:200 1:50-1:100 |
Uniprot #: | SwissProt: Q7KZI7 Human |
Alternative names: | ELKL motif kinase 1 ELKL motif kinase EMK-1 EMK1 MAP/microtubule affinity regulating kinase 2 MAP/microtubule affinity-regulating kinase 2 Mark2 MARK2_HUMAN MGC99619 PAR 1 Par 1b PAR1 homolog Par1b Ser/Thr protein kinase PAR 1B Serine/threonine protein kinase EMK Serine/threonine protein kinase MARK2 Serine/threonine-protein kinase MARK2 |
Fig1: Western blot analysis of MARK2 on MCF-7 (1) and SK-Br-3 (2) cell lysate using anti-MARK2 antibody at 1/2,000 dilution. | |
Fig2: Immunohistochemical analysis of paraffin-embedded human appendix tissue using anti-MARK2 antibody. Counter stained with hematoxylin. | |
Fig3: Flow cytometric analysis of MCF-7 cells with MARK2 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |