MARK2 Recombinant Rabbit Monoclonal Antibody [JE40-97]
cat.: ET7109-20
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JE40-97 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 88 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human MARK2 aa 661-789 / 789. |
| Positive control: | A549 cell lysate, Raji cell lysate, U-87 MG cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate, human appendix tissue, Raji. |
| Subcellular location: | Cell membrane. Cytoplasm. Cytoskeleton. |
| Recommended Dilutions:
WB IHC-P FC |
1:500-1:2,000 1:50-1:200 1:1,000 |
| Uniprot #: | SwissProt: Q7KZI7 Human | Q05512 Mouse | O08679 Rat |
| Alternative names: | ELKL motif kinase 1 ELKL motif kinase EMK-1 EMK1 MAP/microtubule affinity regulating kinase 2 MAP/microtubule affinity-regulating kinase 2 Mark2 MARK2_HUMAN MGC99619 PAR 1 Par 1b PAR1 homolog Par1b Ser/Thr protein kinase PAR 1B Serine/threonine protein kinase EMK Serine/threonine protein kinase MARK2 Serine/threonine-protein kinase MARK2 |
Images
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Fig1:
Western blot analysis of MARK2 on different lysates with Rabbit anti-MARK2 antibody (ET7109-20) at 1/1,000 dilution. Lane 1: A549-WT cell lysate Lane 2: A549-KD MARK2 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 88 kDa Observed band size: 75/80 kDa Exposure time: 1 minute; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7109-20) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of MARK2 on different lysates with Rabbit anti-MARK2 antibody (ET7109-20) at 1/1,000 dilution. Lane 1: Raji cell lysate (10 µg/Lane) Lane 2: U-87 MG cell lysate (10 µg/Lane) Lane 3: Mouse brain tissue lysate (20 µg/Lane) Lane 4: Rat brain tissue lysate (20 µg/Lane) Predicted band size: 88 kDa Observed band size: 75/80 kDa Exposure time: 20 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7109-20) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig3: Immunohistochemical analysis of paraffin-embedded human appendix tissue using anti-MARK2 antibody. Counter stained with hematoxylin. |
|
Fig4:
Flow cytometric analysis of Raji cells labeling MARK2. Cells were fixed and permeabilized. Then stained with the primary antibody (ET7109-20, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.