CBS Recombinant Rabbit Monoclonal Antibody [JE40-99]
cat.: ET7109-21
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB
Clonality: Monoclonal
Clone number: JE40-99
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 61 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human CBS aa 360-551 / 551.
Positive control: 293T (Human embryonic kidney cell) cell lysate, HeLa (Human cervical adenocarcinoma cell) cell lysate, MCF7 (Human breast cancer cell) cell lysate, LNCaP (Human prostate cancer cell) cell lysate.
Subcellular location: Cytoplasm. Nucleus.
Recommended Dilutions:
  WB

1:1,000
Uniprot #: SwissProt: P35520 Human
Alternative names: AI047524 AI303044 Beta thionase Beta-thionase Cbs Cbs cystathionine beta-synthase CBS_HUMAN Cystathionine beta synthase Cystathionine beta-synthase EC 4.2.1.22 HIP 4 HIP4 Methylcysteine synthase MGC18856 MGC18895 MGC37300 OTTHUMP00000109416 OTTHUMP00000109418 Serine sulfhydrase
Images
ET7109-21_1.jpg Fig1: Western blot analysis of CBS on different lysates with Rabbit anti-CBS antibody (ET7109-21) at 1/5,000 dilution.

Lane 1: 293T (Human embryonic kidney cell) cell lysate
Lane 2: HeLa (Human cervical adenocarcinoma cell) cell lysate
Lane 3: MCF7 (Human breast cancer cell) cell lysate
Lane 4: LNCaP (Human prostate cancer cell) cell lysate

Lysates/proteins at 20 µg/Lane.
Exposure time: 59 seconds; ECL: K1801

Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: ET7109-21, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

Predicted band size: 61 kDa
Observed band size: 61 kDa
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.