CBS Recombinant Rabbit Monoclonal Antibody [JE40-99]
cat.: ET7109-21
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | JE40-99 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 61 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human CBS aa 360-551 / 551. |
| Positive control: | 293 cell lysate, human liver tissue lysate, 293T. |
| Subcellular location: | Cytoplasm. Nucleus. |
| Recommended Dilutions:
WB IF-Cell |
1:500-1:2,000 1:50-1:100 |
| Uniprot #: | SwissProt: P35520 Human |
| Alternative names: | AI047524 AI303044 Beta thionase Beta-thionase Cbs Cbs cystathionine beta-synthase CBS_HUMAN Cystathionine beta synthase Cystathionine beta-synthase EC 4.2.1.22 HIP 4 HIP4 Methylcysteine synthase MGC18856 MGC18895 MGC37300 OTTHUMP00000109416 OTTHUMP00000109418 Serine sulfhydrase |
Images
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Fig1:
Western blot analysis of CBS on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7109-21, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: 293 cell lysate Lane 2: Human liver tissue lysate Predicted band size: 61 kDa Observed band size: 59 kDa |
|
Fig2: ICC staining of CBS in 293T cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7109-21, 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.