| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE40-99 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 61 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human CBS aa 360-551 / 551. |
| Positive control: | 293T (Human embryonic kidney cell) cell lysate, HeLa (Human cervical adenocarcinoma cell) cell lysate, MCF7 (Human breast cancer cell) cell lysate, LNCaP (Human prostate cancer cell) cell lysate. |
| Subcellular location: | Cytoplasm. Nucleus. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: P35520 Human |
| Alternative names: | AI047524 AI303044 Beta thionase Beta-thionase Cbs Cbs cystathionine beta-synthase CBS_HUMAN Cystathionine beta synthase Cystathionine beta-synthase EC 4.2.1.22 HIP 4 HIP4 Methylcysteine synthase MGC18856 MGC18895 MGC37300 OTTHUMP00000109416 OTTHUMP00000109418 Serine sulfhydrase |
|
Fig1:
Western blot analysis of CBS on different lysates with Rabbit anti-CBS antibody (ET7109-21) at 1/5,000 dilution. Lane 1: 293T (Human embryonic kidney cell) cell lysate Lane 2: HeLa (Human cervical adenocarcinoma cell) cell lysate Lane 3: MCF7 (Human breast cancer cell) cell lysate Lane 4: LNCaP (Human prostate cancer cell) cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 59 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ET7109-21, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 61 kDa Observed band size: 61 kDa |