Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human, Mouse, Rat |
Applications: | WB, IF-Cell, IF-Tissue, IHC-P, IP |
Clonality: | Monoclonal |
Clone number: | JE41-11 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | 47 kDa |
Isotype: | IgG |
Immunogen: | Recombinant protein within Human BAF57 aa 1-139 / 411. |
Positive control: | SH-SY-5Y, HepG2, SH-SY-5Y, SK-Br-3, rat uterus tissue, human gallbladder tissue, human lung cancer tissue, human colon tissue. |
Subcellular location: | Nucleus. |
Recommended Dilutions:
WB IF-Cell IF-Tissue IHC-P IP |
1:500-1:2,000 1:50-1:200 1:50-1:200 1:50-1:200 1:50-1:200 |
Uniprot #: | SwissProt: Q969G3 Human | O54941 Mouse | Q56A18 Rat |
Alternative names: | BAF57 BRG1 associated factor 57 BRG1-associated factor 57 Chromatin remodeling complex BRG1 associated factor 57 FLJ35648 SMARCE 1 SMARCE1 SMCE1_HUMAN SWI/SNF related matrix associated actin dependent regulator of chromatin e1 SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily e member 1 SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1 |
Fig1: Western blot analysis of BAF57 on SH-SY-5Y cell lysate using anti-BAF57 antibody at 1/1,000 dilution. | |
Fig2: ICC staining BAF57 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. | |
Fig3: ICC staining BAF57 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. | |
Fig4: ICC staining BAF57 in SK-Br-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
Fig5: Immunohistochemical analysis of paraffin-embedded rat uterus tissue using anti-BAF57 antibody. Counter stained with hematoxylin. | |
Fig6: Immunohistochemical analysis of paraffin-embedded human gallbladder tissue using anti-BAF57 antibody. Counter stained with hematoxylin. | |
Fig7: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-BAF57/SMARCE1 antibody. Counter stained with hematoxylin. | |
Fig8: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-BAF57 antibody. Counter stained with hematoxylin. |