BAF57 Recombinant Rabbit Monoclonal Antibody [JE41-11]
cat.: ET7109-23
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IF-Tissue, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | JE41-11 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 47 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human BAF57 aa 1-139 / 411. |
| Positive control: | SH-SY-5Y, HepG2, SH-SY-5Y, SK-Br-3, rat uterus tissue, human gallbladder tissue, human lung cancer tissue, human colon tissue. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue IHC-P IP |
1:500-1:2,000 1:50-1:200 1:50-1:200 1:50-1:200 1:50-1:200 |
| Uniprot #: | SwissProt: Q969G3 Human | O54941 Mouse | Q56A18 Rat |
| Alternative names: | BAF57 BRG1 associated factor 57 BRG1-associated factor 57 Chromatin remodeling complex BRG1 associated factor 57 FLJ35648 SMARCE 1 SMARCE1 SMCE1_HUMAN SWI/SNF related matrix associated actin dependent regulator of chromatin e1 SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily e member 1 SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1 |
Images
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Fig1: ICC staining BAF57 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig2: ICC staining BAF57 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining BAF57 in SK-Br-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: Immunohistochemical analysis of paraffin-embedded rat uterus tissue using anti-BAF57 antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human gallbladder tissue using anti-BAF57 antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-BAF57/SMARCE1 antibody. Counter stained with hematoxylin. |
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Fig7: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-BAF57 antibody. Counter stained with hematoxylin. |
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Fig8:
Western blot analysis of BAF57 on different lysates with Rabbit anti-BAF57 antibody (ET7109-23) at 1/1,000 dilution. Lane 1: MCF-7 (Human breast cancer cell) cell lysate Lane 2: Hela (Human cervical adenocarcinoma cell) cell lysate Lysates/proteins at 10 µg/Lane. Exposure time: 25s seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ET7109-23, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1006), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 47 kDa Observed band size: 55 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.