BAF57 Recombinant Rabbit Monoclonal Antibody [JE41-11]
cat.: ET7109-23
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IF-Tissue, IHC-P, IP
Clonality: Monoclonal
Clone number: JE41-11
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: 47 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human BAF57 aa 1-139 / 411.
Positive control: SH-SY-5Y, HepG2, SH-SY-5Y, SK-Br-3, rat uterus tissue, human gallbladder tissue, human lung cancer tissue, human colon tissue.
Subcellular location: Nucleus.
Recommended Dilutions:
  WB
  IF-Cell
  IF-Tissue
  IHC-P
  IP

1:500-1:2,000
1:50-1:200
1:50-1:200
1:50-1:200
1:50-1:200
Uniprot #: SwissProt: Q969G3 Human | O54941 Mouse | Q56A18 Rat
Alternative names: BAF57 BRG1 associated factor 57 BRG1-associated factor 57 Chromatin remodeling complex BRG1 associated factor 57 FLJ35648 SMARCE 1 SMARCE1 SMCE1_HUMAN SWI/SNF related matrix associated actin dependent regulator of chromatin e1 SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily e member 1 SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1
Images
ET7109-23_1.jpg Fig1: Western blot analysis of BAF57 on SH-SY-5Y cell lysate using anti-BAF57 antibody at 1/1,000 dilution.
ET7109-23_2.jpg Fig2: ICC staining BAF57 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET7109-23_3.jpg Fig3: ICC staining BAF57 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET7109-23_4.jpg Fig4: ICC staining BAF57 in SK-Br-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET7109-23_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded rat uterus tissue using anti-BAF57 antibody. Counter stained with hematoxylin.
ET7109-23_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human gallbladder tissue using anti-BAF57 antibody. Counter stained with hematoxylin.
ET7109-23_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-BAF57/SMARCE1 antibody. Counter stained with hematoxylin.
ET7109-23_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-BAF57 antibody. Counter stained with hematoxylin.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.