Lumican Recombinant Rabbit Monoclonal Antibody [JE11-45]
cat.: ET7109-24
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | JE11-45 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 38 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Lumican aa 172-338 / 338. |
| Positive control: | Jurkat cell lysate, mouse skin tissue lysate, mouse heart tissue lysate, rat skin tissue lysate, MG-63, PANC-1, MCF-7, 293T. |
| Subcellular location: | Secreted, extracellular space, extracellular matrix. |
| Recommended Dilutions:
WB IF-Cell FC |
1:1,000 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: P51884 Human | P51885 Mouse | P51886 Rat |
| Alternative names: | Keratan sulfate proteoglycan lumican KSPG lumican LDC LUM LUM_HUMAN Lumican Lumican precursor SLRR2D |
Images
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Fig1:
Western blot analysis of Lumican on different lysates with Rabbit anti-Lumican antibody (ET7109-24) at 1/1,000 dilution. Lane 1: Jurkat cell lysate (20 µg/Lane) Lane 2: Mouse skin tissue lysate (40 µg/Lane) Lane 3: Mouse heart tissue lysate (40 µg/Lane) Lane 4: Rat skin tissue lysate (40 µg/Lane) Predicted band size: 38 kDa Observed band size: 35-55 kDa Exposure time: Lane 1-2: 9 seconds; Lane 3-4: 3 minutes ; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7109-24) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2: ICC staining of Lumican in MG-63 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7109-24, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig3: ICC staining of Lumican in PANC-1 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7109-24, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig4: ICC staining of Lumican in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7109-24, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig5: Flow cytometric analysis of Lumican was done on 293T cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7109-24, 1/50) (purple). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; yellow). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.