Fbx32 Recombinant Rabbit Monoclonal Antibody [JE41-27]
cat.: ET7109-25
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | JE41-27 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 41 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Fbx32 aa 1-160 / 355. |
| Positive control: | Mouse skeletal muscle tissue lysate, rat skeletal muscle tissue lysate, A431, L6. |
| Subcellular location: | Cytoplasm. Nucleus. |
| Recommended Dilutions:
WB IF-Cell |
1:2,000 1:50-1:200 |
| Uniprot #: | SwissProt: Q969P5 Human | Q9CPU7 Mouse | Q91Z62 Rat |
| Alternative names: | 4833442G10Rik AI430017 Atrogin 1 Atrogin-1 ATROGIN1 Atrophy gene 1 F box only protein 32 F-box only protein 32 F-box protein 32 FBX32_HUMAN fbxo25 FBXO32 FLJ32424 MAFbx MGC108443 MGC137646 MGC33610 Muscle atrophy F box Muscle atrophy F box protein Muscle atrophy F-box protein |
Images
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Fig1:
Western blot analysis of Fbx32 on different lysates with Rabbit anti-Fbx32 antibody (ET7109-25) at 1/2,000 dilution. Lane 1: Mouse skeletal muscle tissue lysate Lane 2: Rat skeletal muscle tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 41 kDa Observed band size: 41 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7109-25) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2: ICC staining of Fbx32 in A431 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7109-25, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig3: ICC staining of Fbx32 in L6 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7109-25, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.