ET7109-26

PEN2 Recombinant Rabbit Monoclonal Antibody [JE41-28]

cat.: ET7109-26

Product Type:	Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity:	Human, Mouse, Rat
Applications:	WB, IF-Cell, IHC-P, FC, IHC-Fr, IF-Tissue
Clonality:	Monoclonal
Clone number:	JE41-28

Form:	Liquid
Storage condition:	Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage buffer:	1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration:	1ug/ul
Purification:	Protein A affinity purified.
Molecular weight:	Predicted band size: 12 kDa
Isotype:	IgG
Immunogen:	Recombinant protein within Human PEN2 aa 1-101 / 101.
Positive control:	Daudi cell lysate, 293T cell lysate, A549 cell lysate, SH-SY5Y cell lysate, HeLa cell lysate, A20 cell lysate, Neuro-2a cell lysate, Mouse spleen tissue lysates, Mouse kidney tissue lysate, Rat lung tissue lysate, A549, SH-SY5Y, human lung carcinoma tissue, human kidney tissue, rat cerebellum tissue, mouse testis tissue, human tonsil tissue.
Subcellular location:	Cell membrane. Endoplasmic reticulum. Golgi apparatus.
Recommended Dilutions: WB IHC-P IF-Cell FC IHC-Fr IF-Tissue	1:500-1:2,000 1:50-1:200 1:50-1:200 1:50-1:100 1:100 1:200
Uniprot #:	SwissProt: Q9NZ42 Human \| Q9CQR7 Mouse \| O88777 Rat
Alternative names:	Gamma secretase subunit PEN 2 Gamma Secretase Subunit PEN2 Gamma-secretase subunit PEN-2 Hematopoietic stem/progenitor cells protein MDS033 MDS033 MSTP064 PEN 2 PEN2_HUMAN Presenilin Enhancer 2 Presenilin enhancer 2 homolog Presenilin enhancer protein 2 PSEN2 psenen

Images

	Fig1: Western blot analysis of PEN2 on different lysates with Rabbit anti-PEN2 antibody (ET7109-26) at 1/2,000 dilution. Lane 1: Daudi (Human Burkitt's lymphoma cell) cell lysate Lane 2: 293T (Human embryonic kidney cell) cell lysate Lane 3: A549 (Human lung adenocarcinoma cell) cell lysate Lane 4: SH-SY5Y (Human neuroblastoma cell) cell lysate Lane 5: HeLa (Human cervical adenocarcinoma cell) cell lysate Lane 6: A20 (Mouse B-cell lymphoma cell) cell lysate Lane 7: Neuro-2a (Mouse brain neuroblastoma cell) cell lysate Lane 8: Mouse spleen tissue lysate Lane 9: Mouse kidney tissue lysate Lane 10: Rat lung tissue lysate Lysates/proteins at 20 µg/Lane. Exposure time: 60 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ET7109-26, 1/2,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 12 kDa Observed band size: 12 kDa
	Fig2: ICC staining of PEN2 in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7109-26, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
	Fig3: ICC staining of PEN2 in SH-SY5Y cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7109-26, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
	Fig4: Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using anti-PEN2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET7109-26, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PEN2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET7109-26, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig6: Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue using anti-PEN2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET7109-26, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig7: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-PEN2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET7109-26, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig8: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PEN2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET7109-26, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig9: Flow cytometric analysis of PEN2 was done on A549 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7109-26, 1/50) (purple). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; yellow).
	Fig10: Immunofluorescence analysis of frozen mouse testis tissue with Rabbit anti-PEN2 antibody (ET7109-26) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ET7109-26, green) at 1/100 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
	Fig11: Immunofluorescence analysis of frozen rat brain tissue with Rabbit anti-PEN2 antibody (ET7109-26) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ET7109-26, green) at 1/100 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
	Fig12: Immunofluorescence analysis of paraffin-embedded mouse testis tissue labeling PEN2 with Rabbit anti-PEN2 antibody (ET7109-26) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ET7109-26, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

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