SFRP4 Recombinant Rabbit Monoclonal Antibody [JE41-41]
cat.: ET7109-27
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, FC
Clonality: Monoclonal
Clone number: JE41-41
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: 40 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human SFRP4 aa 181-320 / 346.
Positive control: Mouse testis tissue, A549, HepG2, SH-SY-5Y.
Subcellular location: Secreted.
Recommended Dilutions:
  WB
  FC

1:500-1:2,000
1:50-1:100
Uniprot #: SwissProt: Q6FHJ7 Human | Q9Z1N6 Mouse | Q9JLS4 Rat
Alternative names: Frizzled protein Frizzled protein human endometrium FRP 4 FRP4 FrpHE human endometrium PYL Secreted frizzled-related protein 4 sFRP-4 Sfrp4 SFRP4_HUMAN
Images
ET7109-27_1.jpg Fig1: Western blot analysis of SFRP4 on different lysates with Rabbit anti-SFRP4 antibody (ET7109-27) at 1/1,000 dilution.

Lane 1: A549
Lane 2: HepG2
lane3: Mouse testis

Lysates/proteins at 15 µg/Lane.
Exposure time: 4 seconds; ECL: K1801

Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: ET7109-27, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

Predicted band size: 40 kDa
Observed band size: 40 kDa
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.