| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JE16-41 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 48 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human DDB2 aa 180-427 / 427. |
| Positive control: | Raji, human tonsil tissue, human liver cancer tissue, human skin tissue, A549. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IHC-P FC |
1:500-1:5,000 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: Q92466 Human |
| Alternative names: | damage-specific DNA binding protein 2 Damage-specific DNA-binding protein 2 DDB p48 subunit Ddb2 DDB2_HUMAN DDBb DNA damage-binding protein 2 UV-damaged DNA-binding protein 2 UV-DDB 2 Xeroderma pigmentosum group E protei |
|
Fig1:
Western blot analysis of DDB2 on Raji (Human Burkitt's lymphoma cell) cell lysate with Rabbit anti-DDB2 antibody (ET7109-29) at 1/5,000 dilution. Lysates/proteins at 15 µg/Lane. Exposure time: 94 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ET7109-29, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 48 kDa Observed band size: 48 kDa |
|
Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-DDB2 antibody. Counter stained with hematoxylin. |
|
Fig3: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-DDB2 antibody. Counter stained with hematoxylin. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-DDB2 antibody. Counter stained with hematoxylin. |