CD79a Recombinant Rabbit Monoclonal Antibody [JE43-50]
cat.: ET7109-33
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Tissue, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JE43-50 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 25 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human CD79a aa 50-100 / 226. |
| Positive control: | Daudi cell lysate, Raji cell lysate, human tonsil tissue, human appendix tissue, human spleen tissue, Daudi, human lymph nodes tissue. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IHC-P IF-Tissue FC |
1:500-1:1,000 1:100-1:400 1:50-1:100 1:50-1:100 |
| Uniprot #: | SwissProt: P11912 Human |
| Alternative names: | B lymphocyte-specific MB1 protein B-cell antigen receptor complex-associated protein alpha chain CD 79a CD79a CD79a antigen (immunoglobulin-associated alpha) CD79A antigen CD79a molecule, immunoglobulin-associated alpha CD79A_HUMAN Ig alpha Ig-alpha IGA IgM-alpha Immunoglobulin-associated alpha Ly54 MB-1 membrane glycoprotein MB1 Membrane-bound immunoglobulin-associated protein Surface IgM-associated protein |
Images
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Fig1:
Western blot analysis of CD79a on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Daudi cell lysate Lane 2: Raji cell lysate Predicted band size: 25 kDa Observed band size: 38 kDa |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-CD79a antibody (ET7109-33) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-33) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human appendix tissue with Rabbit anti-CD79a antibody (ET7109-33) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-33) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-CD79a antibody (ET7109-33) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-33) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5: Flow cytometric analysis of CD79a was done on Daudi cells. The cells were fixed, permeabilized and stained with CD79a antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). After incubation of the primary antibody on room temperature for an hour, the cells was stained with a Alexa Fluor™ 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded human lymph nodes tissue with Rabbit anti-CD79a antibody (ET7109-33) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-33) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.