Brachyury / Bry Recombinant Rabbit Monoclonal Antibody [JE44-11]
cat.: ET7109-35
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P, FC, IP |
| Clonality: | Monoclonal |
| Clone number: | JE44-11 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 47 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Brachyury aa 236-435 / 435. |
| Positive control: | Hela cell lysate, 293 cell lysate, SH-SY5Y cell lysate, mouse embryo notochord tissue, A549. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IHC-P FC IP |
1:500-1:1,000 1:1,000 1:50-1:100 Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: O15178 Human | P20293 Mouse |
| Alternative names: | BRAC_HUMAN Brachyury homolog Brachyury protein Bry MGC104817 Protein T SAVA T T brachyury homolog T brachyury transcription factor T Protein T, brachyury homolog (mouse) TFT Transcription factor T |
Images
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Fig1:
Western blot analysis of Brachyury / Bry on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7109-35, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: 293 cell lysate Lane 3: SH-SY5Y cell lysate |
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Fig2:
Immunohistochemical analysis of paraffin-embedded mouse embryo notochord tissue with Rabbit anti-Brachyury / Bry antibody (ET7109-35) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-35) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Flow cytometric analysis of Brachyury / Bry was done on A549 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7109-35, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.