Ndufs4 Recombinant Rabbit Monoclonal Antibody [JE44-69]
cat.: ET7109-37
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat, Zebrafish
Applications: WB, IP, IHC-P, FC
Clonality: Monoclonal
Clone number: JE44-69
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 20 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human Ndufs4 aa 126-175 / 175.
Positive control: Rat heart tissue lysates, zebrafish tissue lysates, rat uterus tissue, human breast cancer tissue, human small intestine tissue, mouse kidney tissue, 293T.
Subcellular location: Mitochondrion.
Recommended Dilutions:
  WB
  IP
  IHC-P
  FC
1:500-1:1,000
1:10-1:50
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: O43181 Human | Q9CXZ1 Mouse | Q5XIF3 Rat
Alternative names: AQDQ CI 18 CI 18 kDa CI AQDQ CI-18 kDa CI-AQDQ Complex I 18 kDa Complex I AQDQ Complex I-18 kDa Complex I-AQDQ mitochondrial mitochondrial respiratory chain complex I (18 KD subunit) NADH coenzyme Q reductase NADH dehydrogenase (ubiquinone) Fe S protein 4 18kDa NADH dehydrogenase [ubiquinone] iron-sulfur protein 4 NADH dehydrogenase NADH ubiquinone oxidoreductase 18 kDa subunit NADH-ubiquinone oxidoreductase 18 kDa subunit NDUFS4 NDUS4_HUMAN
Images
ET7109-37_1.jpg Fig1: Western blot analysis of Ndufs4 on different lysates with Rabbit anti-Ndufs4 antibody (ET7109-37) at 1/2,000 dilution.

Lane 1: HCT 116-si NT cell lysate
Lane 2: HCT 116-si Ndufs4 cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 20 kDa
Observed band size: 20 kDa

Exposure time: 1 minute; ECL: K1802;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7109-37) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET7109-37_2.jpg Fig2: Western blot analysis of Ndufs4 on zebrafish tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7109-37, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
ET7109-37_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded rat uterus tissue using anti-Ndufs4 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ET7109-37) at 1/100 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.
ET7109-37_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-Ndufs4 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ET7109-37) at 1/100 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.
ET7109-37_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-Ndufs4 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ET7109-37) at 1/100 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.
ET7109-37_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Ndufs4 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ET7109-37) at 1/100 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.
ET7109-37_7.jpg Fig7: Flow cytometric analysis of Ndufs4 was done on 293T cells. The cells were fixed, permeabilized and stained with Ndufs4 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). After incubation of the primary antibody on room temperature for an hour, the cells was stained with a Alexa Fluor™ 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.