RPA32/RPA2 Recombinant Rabbit Monoclonal Antibody [JE45-59]
cat.: ET7109-41
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IP, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE45-59 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 29 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human RPA32 / RPA2 aa 221-270 / 270. |
| Positive control: | Mouse testis tissue, A431, SH-SY-5Y, SiHa, 293, Hela, HepG2, rat brain tissue, human colon tissue, human breast cancer tissue, human kidney tissue. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IP IHC-P |
1:500-1:2,000 1:10-1:50 1:50-1:200 |
| Uniprot #: | SwissProt: P15927 Human | Q62193 Mouse | Q63528 Rat |
| Alternative names: | 60S acidic ribosomal protein P1 AA409079 AI325195 AU020965 ik:tdsubc_2g1 M(2)21C MGC137236 OTTHUMP00000004008 p32 p34 RCJMB04_6d17 replication protein A2, 32kDa REPA2 Replication factor A protein 2 Replication protein A 32 kDa subunit Replication protein A 32kDa subunit Replication protein A 34 kDa subunit Replication protein A Replication Protein A2 (32kDa) Replication protein A2 Replication protein A2, 32kDa RF-A protein 2 Rf-A2 RFA RFA2_HUMAN RP-A p32 RP-A p34 RP21C RPA 2 RPA 32 RPA Rpa2 RPA32 RPA34 RpLP1 RpP2 xx:tdsubc_2g1 zgc:109822 |
Images
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Fig1:
Western blot analysis of RPA32/RPA2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Mouse testis tissue lysate Lane 2: A431 cell lysate Lane 3: SH-SY-5Y cell lysate Lane 4: SiHa cell lysate Lane 5: 293 cell lysate Lane 6: Hela cell lysate Lane 7: HepG2 cell lysate |
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Fig2: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-RPA32/RPA2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ET7109-41) at 1/50 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-RPA32/RPA2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ET7109-41) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-RPA32/RPA2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ET7109-41) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-RPA32/RPA2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ET7109-41) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX. |
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Fig6: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-RPA32/RPA2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ET7109-41) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.