ATP2A1/SERCA1 Recombinant Rabbit Monoclonal Antibody [JE45-75]
cat.: ET7109-43
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IP, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE45-75 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 110 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human SERCA1 ATPase aa 1-50 / 1,001. |
| Positive control: | Human fetal skeletal muscle tissue lysate, human fetal skeletal muscle tissue, human striated muscle tissue. |
| Subcellular location: | Endoplasmic reticulum membrane, Sarcoplasmic reticulum membrane. |
| Recommended Dilutions:
WB IP IHC-P |
1:500-1:1,000 1:10-1:50 1:50-1:400 |
| Uniprot #: | SwissProt: O14983 Human |
| Alternative names: | fast twitch skeletal muscle isoform AT2A1_HUMAN ATP2A ATP2A1 ATPase Ca++ transporting cardiac muscle fast twitch 1 ATPase Ca++ transporting fast twitch 1 ATPase, Ca(2+)-transporting fast twitch 1 Calcium pump 1 Calcium transporting ATPase sarcoplasmic reticulum type fast twitch skeletal muscle isoform Calcium-transporting ATPase sarcoplasmic reticulum type EC 3.6.3.8 Endoplasmic reticulum class 1/2 Ca(2+) ATPase Fast skeletal muscle SR calcium ATPase OTTHUMP00000162561 OTTHUMP00000162562 Sarcoendoplasmic reticulum calcium ATPase Sarcoplasmic reticulum Ca(2+)-ATPase 1 Sarcoplasmic/endoplasmic reticulum calcium ATPase 1 SERCA 1 SERCA1 SERCA1 truncated isoform, included SR Ca(2+) ATPase 1 SR Ca(2+)-ATPase 1 |
Images
|
Fig1: Western blot analysis of SERCA1 ATPase on human fetal skeletal muscle tissue lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. |
|
Fig2: Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-SERCA1 ATPase antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 mins. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ET7109-43) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human striated muscle tissue with Rabbit anti-ATP2A1/SERCA1 antibody (ET7109-43) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-43) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.