RNF40 Recombinant Rabbit Monoclonal Antibody [JE46-30]
cat.: ET7109-46
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IP, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE46-30 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 114 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human RNF40 aa 211-451 / 1,001. |
| Positive control: | HEK-293 cell lysate, HeLa cell lysate, C2C12 cell lysate, MEF cell lysate, Mouse testis tissue lysate, Rat testis tissue lysate, human colon tissue, mouse brain tissue. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IP IHC-P |
1:1,000-1:2,000 1:10-1:50 1:400 |
| Uniprot #: | SwissProt: O75150 Human | Q3U319 Mouse | Q8CJB9 Rat |
| Alternative names: | 95 kDa retinoblastoma associated protein 95 kDa retinoblastoma protein binding protein 95 kDa retinoblastoma-associated protein BRE 1B BRE1 B BRE1 E3 ubiquitin ligase homolog B BRE1-B BRE1B BRE1B_HUMAN DKFZp686K191 E3 ubiquitin protein ligase BRE1B E3 ubiquitin-protein ligase BRE1B KIAA0661 MGC13051 Rb associated protein RBP 95 RBP95 RING finger protein 40 Ring finger protein 40 E3 ubiquitin protein ligase RNF 40 Rnf40 STARING |
Images
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Fig1:
Western blot analysis of RNF40 on different lysates with Rabbit anti-RNF40 antibody (ET7109-46) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate (20 µg/Lane) Lane 2: HeLa cell lysate (20 µg/Lane) Lane 3: C2C12 cell lysate (20 µg/Lane) Lane 4: MEF cell lysate (20 µg/Lane) Lane 5: Mouse testis tissue lysate (40 µg/Lane) Lane 6: Rat testis tissue lysate (40 µg/Lane) Predicted band size: 114 kDa Observed band size: 130 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7109-46) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-RNF40 antibody (ET7109-46) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-46) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-RNF40 antibody (ET7109-46) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-46) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.