SFRS3 Recombinant Rabbit Monoclonal Antibody [JE46-44]
cat.: ET7109-47
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, IF-Cell
Clonality: Monoclonal
Clone number: JE46-44
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 19 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human SFRS3 aa 34-83 / 164.
Positive control: Juekat cell lysate, Hep G2 cell lysate, Hela cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, Mouse testis tissue lysate, rat brain tissue lysate, rat brain tissue, human colon tissue, human breast cancer tissue, human kidney tissue, mouse testis tissue, A431, HeLa..
Subcellular location: Cytoplasm, Nucleus.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
1:500-1:2,000
1:50
1:100-1:500
Uniprot #: SwissProt: P84103 Human | P84104 Mouse
Entrez Gene: 361814 Rat
Alternative names: arginine/serine-rich 3 Pre mRNA splicing factor SRP20 Pre-mRNA-splicing factor SRP20 Serine/arginine-rich splicing factor 3 Splicing factor Splicing factor arginine/serine rich 20 kD Splicing factor arginine/serine rich 3 SRp20 SRSF3 SRSF3_HUMAN X16
Images
ET7109-47_1.jpg Fig1: Western blot analysis of SFRS3 on different lysates with Rabbit anti-SFRS3 antibody (ET7109-47) at 1/5,000 dilution.

Lane 1: Jurkat (Human T-lymphoblastic cells) cell lysate (20 µg/Lane)
Lane 2: Hep G2 (Human hepatocellular carcinoma cell) cell lysate (20 µg/Lane)
Lane 3: HeLa (Human cervical adenocarcinoma cell) cell lysate (20 µg/Lane)
Lane 4: NIH/3T3 (Mouse fibroblast) cell lysate (20 µg/Lane)
Lane 5: PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate (20 µg/Lane)
Lane 6: Mouse brain tissue lysate (40 µg/Lane)
Lane 7: Rat brain tissue lysate (40 µg/Lane)

Exposure time: 60 seconds; ECL: K1801

Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: ET7109-47, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

Predicted band size: 19 kDa
Observed band size: 19 kDa
ET7109-47_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-SFRS3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ET7109-47) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.
ET7109-47_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-SFRS3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ET7109-47) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.
ET7109-47_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-SFRS3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ET7109-47) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.
ET7109-47_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-SFRS3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ET7109-47) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.
ET7109-47_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-SFRS3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ET7109-47) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.
ET7109-47_7.jpg Fig7: Immunocytochemistry analysis of A431 cells labeling SFRS3 with Rabbit anti-SFRS3 antibody (ET7109-47) at 1/50 dilution.

Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SFRS3 antibody (ET7109-47) at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
ET7109-47_8.jpg Fig8: Immunocytochemistry analysis of HeLa cells labeling SFRS3 with Rabbit anti-SFRS3 antibody (ET7109-47) at 1/50 dilution.

Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SFRS3 antibody (ET7109-47) at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.