SAM68 Recombinant Rabbit Monoclonal Antibody [JE47-15]
cat.: ET7109-52
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IHC-P
Clonality: Monoclonal
Clone number: JE47-15
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 48 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human SAM68 aa 1-108 / 443.
Positive control: SH-SY5Y cell lysate, A431 cell lysate, F9 cell lysate, HepG2 cell lysate, A431, human liver cancer tissue, human colon cancer tissue, human stomach cancer tissue.
Subcellular location: Cytoplasm. Membrane. Nucleus.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
1:1,000-1:5,000
1:50
1:1,000
Uniprot #: SwissProt: Q07666 Human | Q3U8T3 Mouse
Alternative names: FLJ34027 GAP associated tyrosine phosphoprotein p62 GAP-associated tyrosine phosphoprotein p62 KH domain containing RNA binding signal transduction associated 1 KH domain-containing KHDR1_HUMAN Khdrbs1 p21 Ras GTPase activating protein associated p62 p21 Ras GTPase-activating protein-associated p62 p62 p68 RNA-binding Sam68 signal transduction-associated protein 1 Src associated in mitosis 68 kDa protein Src-associated in mitosis 68 kDa protein
Images
ET7109-52_1.jpg Fig1: Western blot analysis of SAM68 on different lysates with Rabbit anti-SAM68 antibody (ET7109-52) at 1/500 dilution.

Lane 1: SH-SY5Y cell lysate
Lane 2: A431 cell lysate
Lane 3: F9 cell lysate
Lane 4: HepG2 cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 48 kDa
Observed band size: 69 kDa

Exposure time: 30 seconds;

8% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7109-52) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
ET7109-52_2.jpg Fig2: ICC staining of SAM68 in A431 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7109-52, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
ET7109-52_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-SAM68 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-52, 1/1,000) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7109-52_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-SAM68 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-52, 1/1,000) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7109-52_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-SAM68 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-52, 1/1,000) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.