GNB2 Recombinant Rabbit Monoclonal Antibody [JE47-16]
cat.: ET7109-53
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JE47-16 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 37 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human GNB2 aa 1-130 / 340. |
| Positive control: | MCF-7 cell lysate, SK-Br-3 cell lysate, SH-SY5Y cell lysate, rat kidney tissue, mouse brain tissue, MCF-7. |
| Subcellular location: | Perinuclear region. |
| Recommended Dilutions:
WB IHC-P FC |
1:500-1:1,000 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: P62879 Human | P62880 Mouse | P54313 Rat |
| Alternative names: | G protein beta 2 subunit G protein subunit beta 2 G protein subunit beta-2 GBB2_HUMAN Gnb2 Gnb2l1 Guanine nucleotide binding protein beta 2 subunit Guanine nucleotide binding protein G I G S G T beta 2 subunit 2 Guanine nucleotide binding protein G protein beta polypeptide 2 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-2 OTTHUMP00000174601 OTTHUMP00000174602 RACK1 Receptor for activated C kinase Receptor of activated protein kinase C 1 Signal transducing guanine nucleotide binding regulatory protein beta Transducin beta chain 2 |
Images
|
Fig1:
Western blot analysis of GNB2 on different lysates with Rabbit anti-GNB2 antibody (ET7109-53) at 1/500 dilution. Lane 1: MCF-7 cell lysate Lane 2: SK-Br-3 cell lysate Lane 3: SH-SY5Y cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 37 kDa Observed band size: 35 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7109-53) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig2: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-GNB2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-53, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-GNB2 antibody (ET7109-53) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-53) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4: Flow cytometric analysis of GNB2 was done on MCF-7 cells. The cells were fixed, permeabilized and stained with GNB2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). After incubation of the primary antibody on room temperature for an hour, the cells was stained with a Alexa Fluor™ 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.