GNB2 Recombinant Rabbit Monoclonal Antibody [JE47-16]
cat.: ET7109-53
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | JE47-16 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 37 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human GNB2 aa 1-130 / 340. |
| Positive control: | HeLa cell lysate, MCF7 cell lysate, NIH/3T3 cell lysate, Mouse brain tissue lysate, C6 cell lysate, Rat brain tissue lysate, human brain tissue, mouse brain tissue, rat brain tissue. |
| Subcellular location: | Perinuclear region. |
| Recommended Dilutions:
WB IHC-P IF-Tissue |
1:5,000 1:200 1:100-1:500 |
| Uniprot #: | SwissProt: P62879 Human | P62880 Mouse | P54313 Rat |
| Alternative names: | G protein beta 2 subunit G protein subunit beta 2 G protein subunit beta-2 GBB2_HUMAN Gnb2 Gnb2l1 Guanine nucleotide binding protein beta 2 subunit Guanine nucleotide binding protein G I G S G T beta 2 subunit 2 Guanine nucleotide binding protein G protein beta polypeptide 2 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-2 OTTHUMP00000174601 OTTHUMP00000174602 RACK1 Receptor for activated C kinase Receptor of activated protein kinase C 1 Signal transducing guanine nucleotide binding regulatory protein beta Transducin beta chain 2 |
Images
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Fig1:
Western blot analysis of GNB2 on different lysates with Rabbit anti-GNB2 antibody (ET7109-53) at 1/5,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: MCF7 cell lysate (20 µg/Lane) Lane 3: NIH/3T3 cell lysate (20 µg/Lane) Lane 4: Mouse brain tissue lysate (40 µg/Lane) Lane 5: C6 cell lysate (20 µg/Lane) Lane 6: Rat brain tissue lysate (40 µg/Lane) Predicted band size: 37 kDa Observed band size: 37 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7109-53) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Application: IF-Tissue Species: Mouse Site: brain Sample: Paraffin-embedded section Antibody concentration: 1/100 |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-GNB2 antibody (ET7109-53) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-53) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-GNB2 antibody (ET7109-53) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-53) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-GNB2 antibody (ET7109-53) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-53) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.