NDUFAF1 Recombinant Rabbit Monoclonal Antibody [JE47-18]
cat.: ET7109-54
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE47-18 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 38 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human NDUFAF1 aa 1-190 / 327. |
| Positive control: | K-562 cell lysate, HeLa cell lysate, Jurkat cell lysate, HEK-293 cell lysate, human kidney tissue, human liver tissue. |
| Subcellular location: | Mitochondrion. |
| Recommended Dilutions:
WB IHC-P |
1:2,000 1:200-1:1,000 |
| Uniprot #: | SwissProt: Q9Y375 Human |
| Alternative names: | CGI 65 CGI65 CIA30 CIA30_HUMAN Complex I intermediate associated protein 30 Complex I intermediate associated protein 30 mitochondrial Complex I intermediate-associated protein 30, mitochondrial NADH dehydrogenase (ubiquinone) 1 alpha subcomplex assembly factor 1 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 1 NADH:ubiquinone oxidoreductase complex assembly factor 1 NDUFAF 1 Ndufaf1 antibod |
Images
|
Fig1:
Western blot analysis of NDUFAF1 on different lysates with Rabbit anti-NDUFAF1 antibody (ET7109-54) at 1/2,000 dilution. Lane 1: K-562 cell lysate Lane 2: HeLa cell lysate Lane 3: Jurkat cell lysate Lane 4: HEK-293 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 38 kDa Observed band size: 33 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7109-54) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-NDUFAF1 antibody (ET7109-54) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-54) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-NDUFAF1 antibody (ET7109-54) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-54) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.