| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE47-25 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 23 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human TC10 aa 60-205 / 205. |
| Positive control: | HEK-293 cell lysate, Rat brain tissue lysate. |
| Subcellular location: | Cytoplasm. Membrane. |
| Recommended Dilutions:
WB |
1:1,000-1:5,000 |
| Uniprot #: | SwissProt: P17081 Human | Q9JJL4 Rat |
| Alternative names: | ARHQ Ras homolog gene family member Q RAS like family 7 member A Ras like protein family member 7A Ras like protein TC10 Ras related GTP binding protein TC10 Ras-like protein family member 7A Ras-like protein TC10 RASL7A Rho related GTP binding protein RhoQ Rho-related GTP-binding protein RhoQ Rhoq RHOQ_HUMAN TC10A |
|
Fig1:
Western blot analysis of TC10 on different lysates with Rabbit anti-TC10 antibody (ET7109-55) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate (20 µg/Lane) Lane 2: Rat brain tissue lysate (40 µg/Lane) Predicted band size: 23 kDa Observed band size: 23 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7109-55) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |