TC10 Recombinant Rabbit Monoclonal Antibody [JE47-25]
cat.: ET7109-55
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Rat
Applications: WB
Clonality: Monoclonal
Clone number: JE47-25
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 23 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human TC10 aa 60-205 / 205.
Positive control: HEK-293 cell lysate, Rat brain tissue lysate.
Subcellular location: Cytoplasm. Membrane.
Recommended Dilutions:
  WB

1:1,000-1:5,000
Uniprot #: SwissProt: P17081 Human | Q9JJL4 Rat
Alternative names: ARHQ Ras homolog gene family member Q RAS like family 7 member A Ras like protein family member 7A Ras like protein TC10 Ras related GTP binding protein TC10 Ras-like protein family member 7A Ras-like protein TC10 RASL7A Rho related GTP binding protein RhoQ Rho-related GTP-binding protein RhoQ Rhoq RHOQ_HUMAN TC10A
Images
ET7109-55_1.jpg Fig1: Western blot analysis of TC10 on different lysates with Rabbit anti-TC10 antibody (ET7109-55) at 1/1,000 dilution.

Lane 1: HEK-293 cell lysate (20 µg/Lane)
Lane 2: Rat brain tissue lysate (40 µg/Lane)

Predicted band size: 23 kDa
Observed band size: 23 kDa

Exposure time: 6 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7109-55) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.