TPPP Recombinant Rabbit Monoclonal Antibody [JE47-35]
cat.: ET7109-56
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, FC
Clonality: Monoclonal
Clone number: JE47-35
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 24 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human TPPP aa 80-185 / 219.
Positive control: Mouse brain tissue lysates, rat brain tissue, human pancreas tissue, human uterus tissue, SHG-44.
Subcellular location: Cytoplasm. Cytoskeleton. Nucleus
Recommended Dilutions:
  WB
  IHC-P
  FC
1:1,000-1:2,000
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: O94811 Human | Q7TQD2 Mouse | D3ZQL7 Rat
Alternative names: 25 kDa brain specific protein 25 kDa brain-specific protein Brain specific protein p25 alpha Glycogen synthase kinase 3 (GSK3) inhibitor p24 OTTHUMP00000161630 p24 p25 p25-alpha p25alpha TPPP TPPP/p25 TPPP_HUMAN TPPP1 Tubulin polymerization promoting protein Tubulin polymerization-promoting protein
Images
ET7109-56_1.jpg Fig1: Western blot analysis of TPPP on mouse brain tissue lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7109-56, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
ET7109-56_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-TPPP antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-56, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7109-56_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-TPPP antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-56, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7109-56_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-TPPP antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-56, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7109-56_5.jpg Fig5: Flow cytometric analysis of TPPP was done on SHG-44 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7109-56, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; blue).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.