COPS3 / CSN3 Recombinant Rabbit Monoclonal Antibody [JE47-48]
cat.: ET7109-60
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IHC-P, FC
Clonality: Monoclonal
Clone number: JE47-48
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 48 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human COPS3 / CSN3 aa 280-423 / 423.
Positive control: MCF7 cell lysate, Neuro-2a cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate, Rat skeletal muscle tissue lysate, MCF7, Neuro-2a, human brain tissue, human skeletal mucle tissue, mouse brain tissue, mouse skeletal mucle tissue, rat brain tissue, rat skeletal mucle tissue.
Subcellular location: Cytoplasm. Nucleus.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  FC
1:5,000
1:100
1:1,000
1:1,000
Uniprot #: SwissProt: Q9UNS2 Human | O88543 Mouse | Q68FW9 Rat
Alternative names: Constitutive photomorphogenic homolog subunit 3 COP 9 (constitutive photomorphogenic) subunit 3 COP 9 complex homolog subunit 3 COP 9 complex S3 COP 9 complex subunit 3 COP 9 constitutive photomorphogenic homolog subunit 3 COP 9 homolog COP 9 signalosome complex subunit 3 COP 9 signalosome subunit 3 COP 9 subunit 3 COP9 (constitutive photomorphogenic) subunit 3 COP9 complex homolog subunit 3 COP9 complex S3 COP9 complex subunit 3 COP9 constitutive photomorphogenic homolog subunit 3 COP9 homolog COP9 signalosome complex subunit 3 COP9 signalosome subunit 3 COP9 subunit 3 COPS 3 cops3 CSN 3 CSN3 CSN3_HUMAN JAB 1 containing signalosome subunit 3 JAB1 containing signalosome subunit 3 JAB1-containing signalosome subunit 3 SGN 3 SGN3 Signalosome subunit 3
Images
ET7109-60_1.jpg Fig1: Western blot analysis of COPS3 / CSN3 on different lysates with Rabbit anti-COPS3 / CSN3 antibody (ET7109-60) at 1/5,000 dilution.

Lane 1: MCF7 cell lysate (20 µg/Lane)
Lane 2: Neuro-2a cell lysate (20 µg/Lane)
Lane 3: Mouse brain tissue lysate (40 µg/Lane)
Lane 4: Rat brain tissue lysate (40 µg/Lane)
Lane 5: Rat skeletal muscle tissue lysate (40 µg/Lane)

Predicted band size: 48 kDa
Observed band size: 45 kDa

Exposure time: 10 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7109-60) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET7109-60_2.jpg Fig2: Immunocytochemistry analysis of MCF7 cells labeling COPS3 / CSN3 with Rabbit anti-COPS3 / CSN3 antibody (ET7109-60) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-COPS3 / CSN3 antibody (ET7109-60) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
ET7109-60_3.jpg Fig3: Immunocytochemistry analysis of Neuro-2a cells labeling COPS3 / CSN3 with Rabbit anti-COPS3 / CSN3 antibody (ET7109-60) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-COPS3 / CSN3 antibody (ET7109-60) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
ET7109-60_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-COPS3 / CSN3 antibody (ET7109-60) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-60) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7109-60_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human skeletal mucle tissue with Rabbit anti-COPS3 / CSN3 antibody (ET7109-60) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-60) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7109-60_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-COPS3 / CSN3 antibody (ET7109-60) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-60) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7109-60_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded mouse skeletal mucle tissue with Rabbit anti-COPS3 / CSN3 antibody (ET7109-60) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-60) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7109-60_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-COPS3 / CSN3 antibody (ET7109-60) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-60) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7109-60_9.jpg Fig9: Immunohistochemical analysis of paraffin-embedded rat skeletal mucle tissue with Rabbit anti-COPS3 / CSN3 antibody (ET7109-60) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-60) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7109-60_10.jpg Fig10: Flow cytometric analysis of MCF7 cells labeling COPS3 / CSN3.

Cells were fixed and permeabilized. Then stained with the primary antibody (ET7109-60, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
ET7109-60_11.jpg Fig11: Flow cytometric analysis of Neuro-2a cells labeling COPS3 / CSN3.

Cells were fixed and permeabilized. Then stained with the primary antibody (ET7109-60, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
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