COPS3/CSN3 Recombinant Rabbit Monoclonal Antibody [JE47-48]
cat.: ET7109-60
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IHC-P, FC
Clonality: Monoclonal
Clone number: JE47-48
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 48 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human COPS3 / CSN3 aa 280-423 / 423.
Positive control: Human skeletal muscle tissue, Rat bone marrow, A431, HCT116, rat heart tissue, human liver cancer tissue, human breast tissue, human pancreas tissue, mouse kidney tissue, mouse heart tissue, 293.
Subcellular location: Cytoplasm. Nucleus.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  FC
1:500-1:1,000
1:500-1:2,000
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: Q9UNS2 Human | O88543 Mouse | Q68FW9 Rat
Alternative names: Constitutive photomorphogenic homolog subunit 3 COP 9 (constitutive photomorphogenic) subunit 3 COP 9 complex homolog subunit 3 COP 9 complex S3 COP 9 complex subunit 3 COP 9 constitutive photomorphogenic homolog subunit 3 COP 9 homolog COP 9 signalosome complex subunit 3 COP 9 signalosome subunit 3 COP 9 subunit 3 COP9 (constitutive photomorphogenic) subunit 3 COP9 complex homolog subunit 3 COP9 complex S3 COP9 complex subunit 3 COP9 constitutive photomorphogenic homolog subunit 3 COP9 homolog COP9 signalosome complex subunit 3 COP9 signalosome subunit 3 COP9 subunit 3 COPS 3 cops3 CSN 3 CSN3 CSN3_HUMAN JAB 1 containing signalosome subunit 3 JAB1 containing signalosome subunit 3 JAB1-containing signalosome subunit 3 SGN 3 SGN3 Signalosome subunit 3
Images
ET7109-60_1.jpg Fig1: Western blot analysis of COPS3/CSN3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7109-60, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Human skeletal muscle tissue lysate
Lane 2: Rat bone marrow lysate
ET7109-60_2.jpg Fig2: ICC staining of COPS3/CSN3 in A431 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7109-60, 1/500) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
ET7109-60_3.jpg Fig3: ICC staining of COPS3/CSN3 in HCT116 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7109-60, 1/500) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
ET7109-60_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-COPS3/CSN3 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-60, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7109-60_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-COPS3/CSN3 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-60, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7109-60_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-COPS3/CSN3 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-60, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7109-60_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-COPS3/CSN3 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-60, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7109-60_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-COPS3/CSN3 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-60, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7109-60_9.jpg Fig9: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-COPS3/CSN3 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-60, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7109-60_10.jpg Fig10: Flow cytometric analysis of COPS3/CSN3 was done on 293 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7109-60, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.