BAG2 Recombinant Rabbit Monoclonal Antibody [JE47-57]
cat.: ET7109-62
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, FC |
| Clonality: | Monoclonal |
| Clone number: | JE47-57 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 24 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human BAG2 aa 1-118 / 211. |
| Positive control: | HepG2 cell lysate, Hela cell lysate, Jurkat cell lysate, A549 cell lysate, K562 cell lysate, Hela. |
| Subcellular location: | Cytoskeleton, Cytosol. |
| Recommended Dilutions:
WB FC |
1:500-1:1,000 1:50-1:100 |
| Uniprot #: | SwissProt: O95816 Human |
| Alternative names: | BAG 2 BAG family molecular chaperone regulator 2 BAG-2 BAG-family molecular chaperone regulator-2 Bag2 BAG2_HUMAN BCL 2 associated athanogene 2 Bcl-2-associated athanogene 2 BCL2 associated athanogene 2 BCL2-associated athanogene 2 dJ417I1.2 (BAG family molecular chaperone regulator 2) dJ417I1.2 KIAA0576 MGC149462 OTTHUMP00000016668 |
Images
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Fig1:
Western blot analysis of BAG2 on different lysates with Rabbit anti-BAG2 antibody (ET7109-62) at 1/500 dilution. Lane 1: HepG2 cell lysate Lane 2: Hela cell lysate Lane 3: Jurkat cell lysate Lane 4: A549 cell lysate Lane 5: K562 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 24 kDa Observed band size: 24 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7109-62) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig2: Flow cytometric analysis of BAG2 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7109-62, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.