BAG2 Recombinant Rabbit Monoclonal Antibody [JE47-57]
cat.: ET7109-62
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, FC |
| Clonality: | Monoclonal |
| Clone number: | JE47-57 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 24 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human BAG2 aa 1-118 / 211. |
| Positive control: | Hela cell lysate, Jurkat cell lysate, HEK-293 cell lysate, K-562 cell lysate, Hela. |
| Subcellular location: | Cytoskeleton, Cytosol. |
| Recommended Dilutions:
WB FC |
1:1,000-1:5,000 1:50-1:100 |
| Uniprot #: | SwissProt: O95816 Human |
| Alternative names: | BAG 2 BAG family molecular chaperone regulator 2 BAG-2 BAG-family molecular chaperone regulator-2 Bag2 BAG2_HUMAN BCL 2 associated athanogene 2 Bcl-2-associated athanogene 2 BCL2 associated athanogene 2 BCL2-associated athanogene 2 dJ417I1.2 (BAG family molecular chaperone regulator 2) dJ417I1.2 KIAA0576 MGC149462 OTTHUMP00000016668 |
Images
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Fig1:
Western blot analysis of BAG2 on different lysates with Rabbit anti-BAG2 antibody (ET7109-62) at 1/5,000 dilution. Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate Lane 2: K-562 (Human chronic myelogenous leukemia cell) cell lysate Lane 3: HEK-293 (Human embryonic kidney cell) cell lysate Lane 4: Jurkat (Human T-lymphoblastic cells) cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 46 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ET7109-62, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1006), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 23.8 kDa Observed band size: 24 kDa |
|
Fig2: Flow cytometric analysis of BAG2 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7109-62, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.