ET7109-66

Calpain small subunit 1 Recombinant Rabbit Monoclonal Antibody [JE47-72]

cat.: ET7109-66

Product Type:	Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity:	Human, Mouse, Rat
Applications:	WB, IHC-P, FC
Clonality:	Monoclonal
Clone number:	JE47-72

Form:	Liquid
Storage condition:	Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer:	1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration:	1ug/ul
Purification:	Immunogen affinity purified.
Molecular weight:	Predicted band size: 28 kDa
Isotype:	IgG
Immunogen:	Recombinant protein within Human Calpain small subunit 1 aa 1-140 / 268.
Positive control:	293T cell lysate, A549 cell lysate, mouse lung tissue lysate, rat lung tissue lysate, human liver cancer tissue, human pancreas tissue, mouse liver tissue, mouse kidney tissue, rat uterus tissue, A549.
Subcellular location:	Cytoplasm. Membrane.
Recommended Dilutions: WB IHC-P FC	1:500-1:2,000 1:50-1:200 1:50-1:100
Uniprot #:	SwissProt: P04632 Human \| O88456 Mouse \| Q64537 Rat
Alternative names:	30K Calcium activated neutral proteinase Calcium activated neutral proteinase small subunit Calcium dependent protease small subunit 1 Calcium dependent protease small subunit Calcium-activated neutral proteinase small subunit Calcium-dependent protease small subunit 1 Calcium-dependent protease small subunit Calpain 4 Calpain 4 small subunit (30K) Calpain 4 small subunit Calpain regulatory subunit Calpain S1 Calpain small polypeptide Calpain small subunit 1 Calpain4 CalpainS1 CANP CANP small subunit CANPS CAPN 4 CAPN S1 CAPN4 CAPNS 1 CAPNS Capns1 CDPS CPNS1_HUMAN CSS 1 CSS1

Images

	Fig1: Western blot analysis of Calpain small subunit 1 on different lysates with Rabbit anti-Calpain small subunit 1 antibody (ET7109-66) at 1/1,000 dilution. Lane 1: 293T cell lysate (20 µg/Lane) Lane 2: A549 cell lysate (20 µg/Lane) Lane 3: Mouse lung tissue lysate (40 µg/Lane) Lane 4: Rat lung tissue lysate (40 µg/Lane) Predicted band size: 28 kDa Observed band size: 28 kDa Exposure time: 1 minute 40 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7109-66) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
	Fig2: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Calpain small subunit 1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET7109-66, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig3: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Calpain small subunit 1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET7109-66, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig4: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Calpain small subunit 1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET7109-66, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig5: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Calpain small subunit 1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET7109-66, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig6: Immunohistochemical analysis of paraffin-embedded rat uterus tissue using anti-Calpain small subunit 1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET7109-66, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig7: Flow cytometric analysis of Calpain small subunit 1 was done on A549 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7109-66, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.