BOB1 Recombinant Rabbit Monoclonal Antibody [JE47-78]
cat.: ET7109-68
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JE47-78 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 27 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human BOB1 aa 156-256. |
| Positive control: | Daudi (Human Burkitt's lymphoma cell) cell lysate, Raji (Human Burkitt's lymphoma cell) cell lysate, Ramos (Human Burkitt's lymphoma cell) cell lysate, human B-cell lymphoma tissue, human tonsil tissue, THP-1. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IHC-P FC |
1:5,000 1:200-1:1,000 1:50-1:100 |
| Uniprot #: | SwissProt: Q16633 Human |
| Alternative names: | B cell Oct binding protein 1 B cell specific coactivator OBF 1 B cell specific coactivator OBF1 B-cell-specific coactivator OBF-1 BOB 1 BOB-1 OBF 1 OBF1 OBF1_HUMAN OCA B OCA-B OCAB OCT binding factor 1 OCT-binding factor 1 POU class 2 associating factor 1 POU domain class 2 associating factor 1 POU domain class 2-associating factor 1 Pou2af1 |
Images
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Fig1:
Western blot analysis of BOB1 on different lysates with Rabbit anti-BOB1 antibody (ET7109-68) at 1/5,000 dilution. Lane 1: Daudi (Human Burkitt's lymphoma cell) cell lysate Lane 2: Raji (Human Burkitt's lymphoma cell) cell lysate Lane 3: Ramos (Human Burkitt's lymphoma cell) cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 4 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ET7109-68, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 27 kDa Observed band size: 36 kDa |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human B-cell lymphoma tissue with Rabbit anti-BOB1 antibody (ET7109-68) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-68) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-BOB1 antibody (ET7109-68) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-68) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Flow cytometric analysis of BOB1 was done on THP-1 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7109-68, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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