ENTPD5 Recombinant Rabbit Monoclonal Antibody [JE47-87]
cat.: ET7109-70
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE47-87 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 47 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human ENTPD5 aa 80-220 / 428. |
| Positive control: | Human kidney tissue lysates, human kidney tissue. |
| Subcellular location: | Endoplasmic reticulum. Secreted. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:1,000 1:50 |
| Uniprot #: | SwissProt: O75356 Human | Q9WUZ9 Mouse | Q6P6S9 Rat |
| Alternative names: | CD39 like 4 AI196558 AI987697 CD39 antigen like 4 CD39 antigen-like 4 CD39 like 4 CD39L4 Ectonucleoside triphosphate diphosphohydrolase 5 ENTP5_HUMAN Entpd5 ER UDPase ER-UDPase GDPase ENTPD5 Guanosine-diphosphatase ENTPD5 MGC163357 MGC163359 mNTPase NTPDase 5 Nucleoside diphosphatase PCPH Pcph proto oncogene protein Proto oncogene CPH UDPase ENTPD5 Uridine-diphosphatase ENTPD5 |
Images
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Fig1: Western blot analysis of ENTPD5 on human kidney tissue lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7109-70, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. |
|
Fig2: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ENTPD5 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-70, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.