Tryptophanyl tRNA synthetase / WRS Recombinant Rabbit Monoclonal Antibody [JE48-08]
cat.: ET7109-73
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JE48-08 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 53 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Tryptophanyl tRNA synthetase aa 385-471 / 471. |
| Positive control: | HeLa cell lysates, human lung tissue, A549. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IHC-P FC |
1:500-1:1,000 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: P23381 Human |
| Alternative names: | Gamma 2 GAMMA-2 Gamma2 hWRS IFI 53 IFI53 IFP 53 IFP53 Interferon induced protein 53 Interferon-induced protein 53 SYWC_HUMAN T2-TrpRS TrpRS Tryptophan tRNA ligase, cytoplasmic Tryptophan tRNA ligase 1 cytoplasmic Tryptophan tRNA ligase Tryptophan--tRNA ligase Tryptophanyl tRNA synthetase Tryptophanyl tRNA synthetase cytoplasmic WARS WARS protein WRS |
Images
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Fig1:
Western blot analysis of Tryptophanyl tRNA synthetase / WRS on HeLa cell lysate with Rabbit anti-Tryptophanyl tRNA synthetase / WRS antibody (ET7109-73) at 1/1,000 dilution. Lysates/proteins at 15 µg/Lane. Exposure time: 30 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ET7109-73, 1/1,000 in 5% NFDM/TBST, room temperature for 2 hours Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 53 kDa Observed band size: 53 kDa |
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Fig2: Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-Tryptophanyl tRNA synthetase / WRS antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-73, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Flow cytometric analysis of Tryptophanyl tRNA synthetase / WRS was done on A549 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7109-73, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.