MXD3 / MAD3 Recombinant Rabbit Monoclonal Antibody [JE48-14]
cat.: ET7109-76
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | JE48-14 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 23 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human MXD3 aa 76-206 / 206. |
| Positive control: | MG-63 cell lysate, SK-OV-3 cell lysate, Ramos cell lysate, HeLa cell lysate, Neuro-2a cell lysate, C6 cell lysate, Mouse testis tissue lysate, Rat testis tissue lysate, human testis tissue. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IHC-P IP |
1:2,000-1:5,000 1:1,000 1-2μg/sample |
| Uniprot #: | SwissProt: Q9BW11 Human | Q80US8 Mouse | Q62912 Rat |
| Alternative names: | bHLHc13 Class C basic helix-loop-helix protein 13 FLJ35523 Likely ortholog of mouse Max dimerization protein 3 MAD3_HUMAN MAX dimerization protein 3 Max dimerizer 3 Max-associated protein 3 Max-interacting transcriptional repressor MAD3 MGC2383 mxd3 Myx OTTHUMP00000223596 OTTHUMP00000223598 |
Images
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Fig1:
Western blot analysis of MXD3 / MAD3 on different lysates with Rabbit anti-MXD3 / MAD3 antibody (ET7109-76) at 1/5,000 dilution. Lane 1: MG-63 cell lysate Lane 2: SK-OV-3 cell lysate Lane 3: Ramos cell lysate Lane 4: HeLa cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 23 kDa Observed band size: 27 kDa Exposure time: 8 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7109-76) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of MXD3 / MAD3 on different lysates with Rabbit anti-MXD3 / MAD3 antibody (ET7109-76) at 1/2,000 dilution. Lane 1: Neuro-2a cell lysate Lane 2: C6 cell lysate Lane 3: Mouse testis tissue lysate Lane 4: Rat testis tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 23 kDa Observed band size: 27 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7109-76) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-MXD3 / MAD3 antibody (ET7109-76) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-76) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
MXD3 / MAD3 was immunoprecipitated from 0.2 mg SK-OV-3 cell lysate with ET7109-76 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using ET7109-76 at 1/2,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: SK-OV-3 cell lysate (input) Lane 2: ET7109-76 IP in SK-OV-3 cell lysate Lane 3: Rabbit IgG instead of ET7109-76 in SK-OV-3 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 1 minute 50 seconds; ECL: K1801 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.