MXD3 / MAD3 Recombinant Rabbit Monoclonal Antibody [JE48-14]
cat.: ET7109-76
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC |
| Clonality: | Monoclonal |
| Clone number: | JE48-14 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 23 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human MXD3 aa 76-206 / 206. |
| Positive control: | Neuro-2a cell lysate, C6 cell lysate, mouse testis tissue lysate, rat testis tissue lysate, SH-SY5Y cell lysate, Hela cell lysate, SHG-44. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB FC |
1:500-1:2,000 1:50-1:100 |
| Uniprot #: | SwissProt: Q9BW11 Human | Q80US8 Mouse | Q62912 Rat |
| Alternative names: | bHLHc13 Class C basic helix-loop-helix protein 13 FLJ35523 Likely ortholog of mouse Max dimerization protein 3 MAD3_HUMAN MAX dimerization protein 3 Max dimerizer 3 Max-associated protein 3 Max-interacting transcriptional repressor MAD3 MGC2383 mxd3 Myx OTTHUMP00000223596 OTTHUMP00000223598 |
Images
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Fig1:
Western blot analysis of MXD3 / MAD3 on different lysates with Rabbit anti-MXD3 / MAD3 antibody (ET7109-76) at 1/2,000 dilution. Lane 1: Neuro-2a cell lysate Lane 2: C6 cell lysate Lane 3: Mouse testis tissue lysate Lane 4: Rat testis tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 23 kDa Observed band size: 27 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7109-76) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of MXD3 / MAD3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7109-76, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SH-SY5Y cell lysate Lane 2: Hela cell lysate |
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Fig3: Flow cytometric analysis of MXD3 / MAD3 was done on SHG-44 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7109-76, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.