TCP1 alpha / CCTA Recombinant Rabbit Monoclonal Antibody [JE48-48]
cat.: ET7109-79
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P, IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | JE48-48 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 60 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human TCP1 alpha / CCTA aa 1-97 / 556. |
| Positive control: | Daudi cell lysates, NIH/3T3 cell lysate, Mouse testis tissue lysate, human liver cancer tissue, human testis tissue, Jurkat. |
| Subcellular location: | Cytoplasm. Cytoskeleton. |
| Recommended Dilutions:
WB IHC-P IF-Cell FC |
1:500-1:1,000 1:200-1:1,000 1:100 1,000 |
| Uniprot #: | SwissProt: P17987 Human | P11983 Mouse |
| Alternative names: | AI528772 c-cpn CCT alpha CCT CCT-alpha CCT1 Ccta CCTalpha D6S230E MGC133746 p63 T complex 1 T complex protein 1 alpha subunit T complex protein 1 T-complex homolog TCP1 T-complex protein 1 subunit alpha T-complex protein 1 subunit alpha B Tailless complex polypeptide 1 Tailless complex polypeptide 1A Tailless complex polypeptide 1B TCP 1 alpha Tcp-1 TCP-1-alpha TCP1 TCPA_HUMAN Tp63 TRic |
Images
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Fig1: Western blot analysis of TCP1 alpha / CCTA on Daudi cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7109-79, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of TCP1 alpha / CCTA on different lysates with Rabbit anti-TCP1 alpha / CCTA antibody (ET7109-79) at 1/5,000 dilution. Lane 1: NIH/3T3 cell lysate Lane 2: Mouse testis tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 60 kDa Observed band size: 60 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7109-79) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue with Rabbit anti-TCP1 alpha / CCTA antibody (ET7109-79) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-79) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-TCP1 alpha / CCTA antibody (ET7109-79) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-79) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunocytochemistry analysis of Jurkat cells labeling TCP1 alpha / CCTA with Rabbit anti-TCP1 alpha / CCTA antibody (ET7109-79) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-TCP1 alpha / CCTA antibody (ET7109-79) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig6:
Flow cytometric analysis of Jurkat cells labeling TCP1 alpha / CCTA. Cells were fixed and permeabilized. Then stained with the primary antibody (ET7109-79, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.