FBP1 Recombinant Rabbit Monoclonal Antibody [JE48-70]
cat.: ET7109-82
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IHC-P
Clonality: Monoclonal
Clone number: JE48-70
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 37 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human FBP1 aa 244-338 / 338.
Positive control: Human liver tissue lysate, human lung tissue lysate, MCF7 cell lysates, PANC-1, rat urinary bladder tissue, human kidney tissue, human small intestine tissue, human pancreas tissue, mouse liver tissue.
Subcellular location: Cytoplasm. Nucleus. Extracellular exosome.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
1:1,000-1:2,000
1:50-1:200
1:50-1:200
Uniprot #: SwissProt: P09467 Human | Q9QXD6 Mouse | P19112 Rat
Alternative names: 6-bisphosphatase 1 6-bisphosphate 1-phosphohydrolase 1 D fructose 1 6 bisphosphate 1 phosphohydrolase 1 D-fructose-1 EC 3.1.3.11 F16P1_HUMAN FBP FBP 1 FBP1 FBPase 1 Fructose 1 6 bisphosphatase 1 Fructose bisphosphatase 1 Fructose-1 Growth inhibiting protein 17 Liver fructose bisphosphatase
Images
ET7109-82_1.jpg Fig1: Western blot analysis of FBP1 on different lysates with Rabbit anti-FBP1 antibody (ET7109-82) at 1/1,000 dilution.

Lane 1: Human liver tissue lysate
Lane 2: Human lung tissue lysate

Lysates/proteins at 30 µg/Lane.

Predicted band size: 37 kDa
Observed band size: 37 kDa

Exposure time: 7 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7109-82) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
ET7109-82_2.jpg Fig2: Western blot analysis of FBP1 on MCF7 cell lysates with Rabbit anti-FBP1 antibody (ET7109-82) at 1/2,000 dilution.

Lysates/proteins at 20 µg/Lane.

Predicted band size: 37 kDa
Observed band size: 37 kDa

Exposure time: 6 seconds; ECL: K1801;
4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7109-82) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET7109-82_3.jpg Fig3: ICC staining of FBP1 in PANC-1 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7109-82, 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
ET7109-82_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded rat urinary bladder tissue using anti-FBP1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-82, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7109-82_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-FBP1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-82, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7109-82_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-FBP1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-82, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7109-82_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-FBP1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-82, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7109-82_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-FBP1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-82, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.