RSK1 p90 Recombinant Rabbit Monoclonal Antibody [JE48-76]
cat.: ET7109-83
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IHC-P, FC
Clonality: Monoclonal
Clone number: JE48-76
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 83 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human RSK1 p90 aa 1-107 / 735.
Positive control: HeLa cell lysate, MCF7 cell lysate, K-562 cell lysate, Daudi cell lysate, PC-12 cell lysate, mouse brain tissue lysate, Hela, human testis tissue, human appendix tissue, human prostate cancer tissue, human small intestine tissue, mouse colon tissue, rat testis tissue.
Subcellular location: Cytoplasm. Nucleus.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  FC
1:500-1:2,000
1:50
1:50-1:200
1:1,000
Uniprot #: SwissProt: Q15418 Human | P18653 Mouse | Q63531 Rat
Alternative names: 90 kDa ribosomal protein S6 kinase 1 dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1 dJ590P13.1 EC 2.7.11.1 HU 1 HU1 KS6A1_HUMAN MAP kinase activated protein kinase 1a MAP kinase-activated protein kinase 1a MAPK-activated protein kinase 1a MAPKAP kinase 1a MAPKAPK-1a MAPKAPK1A MGC79981 Mitogen-activated protein kinase-activated protein kinase 1A OTTHUMP00000004113 p90 RSK1 p90-RSK 1 p90rsk p90RSK1 p90S6K pp90RSK1 Ribosomal protein S6 kinase 90kD 1 Ribosomal protein S6 kinase 90kD polypeptide 1 Ribosomal protein S6 kinase 90kDa polypeptide 1 Ribosomal protein S6 kinase alpha 1 Ribosomal protein S6 kinase alpha-1 Ribosomal protein S6 kinase polypeptide 1 Ribosomal S6 kinase 1 RPS6K1 alpha rps6ka Rps6ka1 RSK 1 RSK 1 p90 RSK RSK-1 RSK1 S6K alpha 1 S6K-alpha-1
Images
ET7109-83_1.jpg Fig1: Western blot analysis of RSK1 p90 on different lysates with Rabbit anti-RSK1 p90 antibody (ET7109-83) at 1/2,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: MCF7 cell lysate
Lane 3: K-562 cell lysate
Lane 4: Daudi cell lysate
Lane 5: PC-12 cell lysate
Lane 6: Mouse brain tissue lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 83 kDa
Observed band size: 75 kDa

Exposure time: 3 minutes; ECL: K1802;
4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7109-83) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET7109-83_2.jpg Fig2: ICC staining of RSK1 p90 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7109-83, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
ET7109-83_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-RSK1 p90 antibody (ET7109-83) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-83) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7109-83_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human appendix tissue using anti-RSK1 p90 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-83, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7109-83_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-RSK1 p90 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-83, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7109-83_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-RSK1 p90 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-83, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7109-83_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-RSK1 p90 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-83, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7109-83_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-RSK1 p90 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-83, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7109-83_9.jpg Fig9: Flow cytometric analysis of HeLa cells labeling RSK1 p90.

Cells were fixed and permeabilized. Then stained with the primary antibody (ET7109-83, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
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