RSK1 p90 Recombinant Rabbit Monoclonal Antibody [JE48-76]
cat.: ET7109-83
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IHC-P, FC
Clonality: Monoclonal
Clone number: JE48-76
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 83 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human RSK1 p90 aa 1-107 / 735.
Positive control: Daudi cell lysates, Hela, rat testis tissue, human appendix tissue, human prostate cancer tissue, human small intestine tissue, mouse colon tissue, F9.
Subcellular location: Cytoplasm. Nucleus.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  FC
1:500-1:2,000
1:50
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: Q15418 Human | P18653 Mouse | Q63531 Rat
Alternative names: 90 kDa ribosomal protein S6 kinase 1 dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1 dJ590P13.1 EC 2.7.11.1 HU 1 HU1 KS6A1_HUMAN MAP kinase activated protein kinase 1a MAP kinase-activated protein kinase 1a MAPK-activated protein kinase 1a MAPKAP kinase 1a MAPKAPK-1a MAPKAPK1A MGC79981 Mitogen-activated protein kinase-activated protein kinase 1A OTTHUMP00000004113 p90 RSK1 p90-RSK 1 p90rsk p90RSK1 p90S6K pp90RSK1 Ribosomal protein S6 kinase 90kD 1 Ribosomal protein S6 kinase 90kD polypeptide 1 Ribosomal protein S6 kinase 90kDa polypeptide 1 Ribosomal protein S6 kinase alpha 1 Ribosomal protein S6 kinase alpha-1 Ribosomal protein S6 kinase polypeptide 1 Ribosomal S6 kinase 1 RPS6K1 alpha rps6ka Rps6ka1 RSK 1 RSK 1 p90 RSK RSK-1 RSK1 S6K alpha 1 S6K-alpha-1
Images
ET7109-83_1.jpg Fig1: Western blot analysis of RSK1 p90 on Daudi cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7109-83, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
ET7109-83_2.jpg Fig2: ICC staining of RSK1 p90 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7109-83, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
ET7109-83_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-RSK1 p90 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-83, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7109-83_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human appendix tissue using anti-RSK1 p90 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-83, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7109-83_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-RSK1 p90 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-83, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7109-83_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-RSK1 p90 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-83, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7109-83_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-RSK1 p90 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-83, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7109-83_8.jpg Fig8: Flow cytometric analysis of RSK1 p90 was done on F9 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7109-83, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
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