CLSTN1 Recombinant Rabbit Monoclonal Antibody [JE48-81]
cat.: ET7109-84
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB
Clonality: Monoclonal
Clone number: JE48-81
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 110 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human CLSTN1 aa 860-981 / 981.
Positive control: A431 cell lysate, SiHa cell lysate, U-87 MG cell lysate, Neuro-2a cell lysate, C6 cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate.
Subcellular location: Endoplasmic reticulum. Golgi apparatus. Membrane. Nucleus.
Recommended Dilutions:
  WB

1:5,000
Uniprot #: SwissProt: O94985 Human | Q9EPL2 Mouse | Q6Q0N0 Rat
Alternative names: Alc alpha Alc-alpha Alcadein alpha 1 Alcadein alpha Alcadein-alpha alcalpha1 alcalpha2 Alzheimer related cadherin like protein Alzheimer-related cadherin-like protein C-terminal fragment 1-alpha Cadherin related family member 12 Calsyntenin 1 Calsyntenin1 CDHR12 CLSTN 1 Clstn1 CS1 CSTN1 CSTN1_HUMAN CTF1-alpha FLJ32258 KIAA0911 Non classical cadherin XB31alpha Non classical cadherin XB31alpha1 Non-classical cadherin XB31alpha PIK3CD SAlc-alpha XB31alpha
Images
ET7109-84_1.jpg Fig1: Western blot analysis of CLSTN1 on different lysates with Rabbit anti-CLSTN1 antibody (ET7109-84) at 1/5,000 dilution.

Lane 1: A431 cell lysate (20 µg/Lane)
Lane 2: SiHa cell lysate (20 µg/Lane)
Lane 3: U-87 MG cell lysate (20 µg/Lane)
Lane 4: Neuro-2a cell lysate (20 µg/Lane)
Lane 5: C6 cell lysate (20 µg/Lane)
Lane 6: Mouse brain tissue lysate (40 µg/Lane)
Lane 7: Rat brain tissue lysate (40 µg/Lane)

Predicted band size: 110 kDa
Observed band size: 110 kDa

Exposure time: 20 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7109-84) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.