Anti-CLSTN1 antibody [JE48-81]
cat.: ET7109-84
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse
Applications: WB, IHC-P
Clonality: Monoclonal
Clone number: JE48-81
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein affinity purified.
Molecular weight: Predicted band size: 110 kDa.
Isotype: IgG
Immunogen: Recombinant protein within human CLSTN1 aa 881-981 (Cytoplasmic).
Positive control: Mouse brain tissue lysate, Siha cell lysate, mouse cerebellum tissue lysate, A431 cell lysate, human liver cancer tissue, human kidney tissue.
Subcellular location: Endoplasmic reticulum. Golgi apparatus. Membrane. Nucleus.
Recommended Dilutions:
  WB
  IHC-P

1:1000-1:5000
1:50-1:200
Uniprot #: SwissProt: O94985 Human | Q9EPL2 Mouse
Alternative names: Alc alpha Alc-alpha Alcadein alpha 1 Alcadein alpha Alcadein-alpha alcalpha1 alcalpha2 Alzheimer related cadherin like protein Alzheimer-related cadherin-like protein C-terminal fragment 1-alpha Cadherin related family member 12 Calsyntenin 1 Calsyntenin1 CDHR12 CLSTN 1 Clstn1 CS1 CSTN1 CSTN1_HUMAN CTF1-alpha FLJ32258 KIAA0911 Non classical cadherin XB31alpha Non classical cadherin XB31alpha1 Non-classical cadherin XB31alpha PIK3CD SAlc-alpha XB31alpha
Images
ET7109-84_1.jpg Fig1: Western blot analysis of CLSTN1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7109-84, 1/2000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Mouse brain tissue lysate
Lane 2: Siha cell lysate
Lane 3: Mouse cerebellum tissue lysate
Lane 4: A431 cell lysate
ET7109-84_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-CLSTN1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-84, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7109-84_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-CLSTN1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-84, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.