| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE48-81 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 110 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human CLSTN1 aa 860-981 / 981. |
| Positive control: | A431 cell lysate, SiHa cell lysate, U-87 MG cell lysate, Neuro-2a cell lysate, C6 cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate. |
| Subcellular location: | Endoplasmic reticulum. Golgi apparatus. Membrane. Nucleus. |
| Recommended Dilutions:
WB |
1:5,000 |
| Uniprot #: | SwissProt: O94985 Human | Q9EPL2 Mouse | Q6Q0N0 Rat |
| Alternative names: | Alc alpha Alc-alpha Alcadein alpha 1 Alcadein alpha Alcadein-alpha alcalpha1 alcalpha2 Alzheimer related cadherin like protein Alzheimer-related cadherin-like protein C-terminal fragment 1-alpha Cadherin related family member 12 Calsyntenin 1 Calsyntenin1 CDHR12 CLSTN 1 Clstn1 CS1 CSTN1 CSTN1_HUMAN CTF1-alpha FLJ32258 KIAA0911 Non classical cadherin XB31alpha Non classical cadherin XB31alpha1 Non-classical cadherin XB31alpha PIK3CD SAlc-alpha XB31alpha |
|
Fig1:
Western blot analysis of CLSTN1 on different lysates with Rabbit anti-CLSTN1 antibody (ET7109-84) at 1/5,000 dilution. Lane 1: A431 cell lysate (20 µg/Lane) Lane 2: SiHa cell lysate (20 µg/Lane) Lane 3: U-87 MG cell lysate (20 µg/Lane) Lane 4: Neuro-2a cell lysate (20 µg/Lane) Lane 5: C6 cell lysate (20 µg/Lane) Lane 6: Mouse brain tissue lysate (40 µg/Lane) Lane 7: Rat brain tissue lysate (40 µg/Lane) Predicted band size: 110 kDa Observed band size: 110 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7109-84) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |