ENTPD5 Recombinant Rabbit Monoclonal Antibody [JE48-94]
cat.: ET7109-86
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | JE48-94 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 48 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human ENTPD5 aa 280-420 / 428. |
| Positive control: | Rat testis tissue, human liver cancer tissue, human kidney tissue, mouse colon tissue, Mouse liver tissue lysate, rat kidney tissue lysate. |
| Subcellular location: | Endoplasmic reticulum. Secreted. |
| Recommended Dilutions:
WB IHC-P IP |
1:500-1:1,000 1:50-1:200 1:50 |
| Uniprot #: | SwissProt: O75356 Human | Q9WUZ9 Mouse | Q6P6S9 Rat |
| Alternative names: | CD39 like 4 AI196558 AI987697 CD39 antigen like 4 CD39 antigen-like 4 CD39 like 4 CD39L4 Ectonucleoside triphosphate diphosphohydrolase 5 ENTP5_HUMAN Entpd5 ER UDPase ER-UDPase GDPase ENTPD5 Guanosine-diphosphatase ENTPD5 MGC163357 MGC163359 mNTPase NTPDase 5 Nucleoside diphosphatase PCPH Pcph proto oncogene protein Proto oncogene CPH UDPase ENTPD5 Uridine-diphosphatase ENTPD5 |
Images
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Fig1:
Western blot analysis of ENTPD5 on different lysates with Rabbit anti-ENTPD5 antibody (ET7109-86) at 1/5,000 dilution. Lane 1: Mouse liver tissue lysate Lane 2: rat kidney tissue lysate Lysates/proteins at 40 µg/Lane. Exposure time: 12 seconds ; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ET7109-86, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 48 kDa Observed band size: 48 kDa |
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Fig2: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-ENTPD5 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-86, 1/50) for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-ENTPD5 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-86, 1/200) for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ENTPD5 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-86, 1/200) for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-ENTPD5 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-86, 1/50) for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.