ET7109-88

Synapsin II Recombinant Rabbit Monoclonal Antibody [JE49-03]

cat.: ET7109-88

Product Type:	Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity:	Mouse, Rat, Human
Applications:	WB, IF-Tissue, IHC-P, FC
Clonality:	Monoclonal
Clone number:	JE49-03

Form:	Liquid
Storage condition:	Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer:	1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration:	1ug/ul
Purification:	Protein A affinity purified.
Molecular weight:	Predicted band size: 63 kDa
Isotype:	IgG
Immunogen:	Recombinant protein within Human Synapsin II aa 180-320 / 582.
Positive control:	Rat bone marrow tissue, rat brain tissue, mouse brain tissue, F9, rat cerebellum tissue.
Subcellular location:	Cell junction. Synapse.
Recommended Dilutions: WB IF-Tissue IHC-P FC	1:500 1:50-1:200 1:50-1:200 1:50-1:100
Uniprot #:	SwissProt: Q92777 Human \| Q64332 Mouse \| Q63537 Rat
Alternative names:	SYN 2 SYN II SYN IIa SYN IIb SYN2 Synapsin 2 Synapsin II isoform IIa Synapsin II isoform IIb Synapsin2 SynapsinII SYNII SYNIIa SYNIIb

Images

	Fig1: IF staining of Synapsin II in rat bone marrow (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.V
	Fig2: Immunofluorescence analysis of paraffin-embedded rat brain tissue labeling Synapsin II (ET7109-88) and GFAP (EM140707). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Synapsin II (ET7109-88, red) at 1/200 dilution and GFAP (EM140707, green) at 1/400 dilution overnight at 4 ℃, washed with PBS. iFluor™ 594 conjugate-Goat anti-Rabbit IgG (HA1122) and iFluor™ 488 conjugate-Goat anti-Mouse IgG (HA1125) were used as the secondary antibodies at 1/1,000 dilution. DAPI was used as nuclear counterstain.
	Fig3: Immunofluorescence analysis of paraffin-embedded mouse brain tissue labeling Synapsin II (ET7109-88) and GFAP (EM140707). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Synapsin II (ET7109-88, red) at 1/200 dilution and GFAP (EM140707, green) at 1/400 dilution overnight at 4 ℃, washed with PBS. iFluor™ 594 conjugate-Goat anti-Rabbit IgG (HA1122) and iFluor™ 488 conjugate-Goat anti-Mouse IgG (HA1125) were used as the secondary antibodies at 1/1,000 dilution. DAPI was used as nuclear counterstain.

	Fig4: Immunohistochemical analysis of paraffin-embedded rat bone marrow tissue using anti-Synapsin II antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET7109-88, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig5: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Synapsin II antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET7109-88, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Synapsin II antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET7109-88, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig7: Flow cytometric analysis of Synapsin II was done on F9 cells. The cells were fixed, permeabilized and stained with Synapsin II antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). After incubation of the primary antibody on room temperature for an hour, the cells was stained with a Alexa Fluor™ 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.

Fig8: Immunofluorescence analysis of paraffin-embedded rat cerebellum tissue labeling Synapsin II (ET7109-88) and GFAP (EM140707).

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Synapsin II (ET7109-88, red) at 1/200 dilution and GFAP (EM140707, green) at 1/400 dilution overnight at 4 ℃, washed with PBS.

iFluor™ 594 conjugate-Goat anti-Rabbit IgG (HA1122) and iFluor™ 488 conjugate-Goat anti-Mouse IgG (HA1125) were used as the secondary antibodies at 1/1,000 dilution. DAPI was used as nuclear counterstain.

Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.