APRIL/TNFSF13 Recombinant Rabbit Monoclonal Antibody [JE49-07]
cat.: ET7109-89
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | JE49-07 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 27 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human APRIL / TNFSF13 aa 141-250 / 250. |
| Positive control: | Hela cell and A549 cell lysates, A549. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB IF-Cell |
1:500-1:1,000 1:50 |
| Uniprot #: | SwissProt: O75888 Human |
| Alternative names: | A proliferation inducing ligand A proliferation-inducing ligand APRIL CD256 TALL-2 TALL2 TNF related death ligand TNF- and APOL-related leukocyte expressed ligand 2 TNF-related death ligand 1 TNF13_HUMAN TNFSF13 TRDL-1 TRDL1 Tumor necrosis factor (ligand) superfamily, member 13 Tumor necrosis factor ligand superfamily member 13 Tumor necrosis factor like protein ZTNF2 Tumor necrosis factor related death ligand 1 UNQ383/PRO715 ZTNF2 |
Images
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Fig1:
Western blot analysis of APRIL/TNFSF13 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7109-89, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Lane 1: Hela cell lysate Lane 2: A549 cell lysate Predicted band size: 27 kDa Observed band size: 14 kDa |
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Fig2: ICC staining of APRIL/TNFSF13 in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7109-89, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.