ICAD Recombinant Rabbit Monoclonal Antibody [JE49-23]
cat.: ET7109-91
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | JE49-23 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 37 kDa. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human ICAD aa 37-137. |
| Positive control: | Jurkat cell lysate, human stomach tissue lysate, human thyroid tissue, human breast tissue, human small intestine tissue, human pancreas intestine tissue, HCT116. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IHC-P FC |
1:500-1:1000 1:200-1:500 1:50-1:100 |
| Uniprot #: | SwissProt: O00273 Human |
| Alternative names: | A330085O09Rik Caspase activated deoxyribonuclease inhibitor short form DFF 1 DFF 45 DFF alpha DFF-45 DFF1 DFF35 DFF45 DFFA Dffa DNA fragmentation factor, alpha subunit DFFA_HUMAN DNA fragmentation factor 45 kDa subunit DNA Fragmentation Factor Alpha Subunit DNA fragmentation factor subunit alpha DNA fragmentation factor, 45 kD, alpha subunit DNA fragmentation factor, 45kDa, alpha polypeptide (DFFA), transcript variant 1 DNA fragmentation factor, 45kDa, alpha polypeptide DNA fragmentation factor, alpha subunit DNAation factor 45 kDa subunit H13 ICAD ICAD L ICAD S Inhibitor of CAD Inhibitor of Caspase Activated DNase MGC143066 OTTHUMP00000001903 OTTHUMP00000001904 RP23 121D17.3 |
Images
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Fig1:
Western blot analysis of ICAD on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7109-91, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Jurkat cell lysate Lane 2: Human stomach tissue lysate Predicted band size: 37 kDa Observed band size: 55 kDa |
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Fig2: Immunohistochemical analysis of paraffin-embedded human thyroid tissue using anti-ICAD antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-91, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-ICAD antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-91, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-ICAD antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-91, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human pancreas intestine tissue using anti-ICAD antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-91, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6: Flow cytometric analysis of ICAD was done on HCT116 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7109-91, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.