PKA R2 Recombinant Rabbit Monoclonal Antibody [JE49-49]
cat.: ET7109-92
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, IHC-P, IP
Clonality: Monoclonal
Clone number: JE49-49
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 46 kDa.
Isotype: IgG
Immunogen: Recombinant protein within Human PKA R2 aa 272-404 / 404.
Positive control: Hela cell lysates, human liver cancer tissue, human skin tissue, human breast tissue, human placenta tissue.
Subcellular location: Cell membrane, Cytoplasm, Membrane.
Recommended Dilutions:
  WB
  IHC-P
  IP
1:500-1:1,000
1:50-1:200
1:10-1:50
Uniprot #: SwissProt: P13861 Human
Alternative names: Alternative names cAMP dependent protein kinase regulatory subunit alpha 2 cAMP dependent protein kinase regulatory subunit RII alpha cAMP dependent protein kinase type II alpha regulatory chain cAMP dependent protein kinase type II alpha regulatory subunit cAMP-dependent protein kinase type II-alpha regulatory subunit KAP2 KAP2_HUMAN MGC3606 PKR 2 PKR2 PRKA R2 PRKAR 2 PRKAR2 PRKAR2A Protein kinase A RII alpha subunit Protein kinase cAMP dependent regulatory type II alpha
Images
ET7109-92_1.jpg Fig1: Western blot analysis of PKA R2 on different lysates with Rabbit anti-PKA R2 antibody (ET7109-92) at 1/1,000 dilution.

Lane 1: A549-WT cell lysate
Lane 2: A549-KD PKA R2 cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 46 kDa
Observed band size: 46 kDa

Exposure time: 30 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7109-92) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET7109-92_2.jpg Fig2: Western blot analysis of PKA R2 on Hela cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7109-92, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
ET7109-92_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-PKA R2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-92, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7109-92_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-PKA R2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-92, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7109-92_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-PKA R2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-92, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7109-92_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-PKA R2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-92, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.