EWSR1/EWS Recombinant Rabbit Monoclonal Antibody [JE49-57]
cat.: ET7109-94
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IHC-P
Clonality: Monoclonal
Clone number: JE49-57
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 68 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human EWSR1 / EWS aa 1-229 / 656.
Positive control: K-562 (Human chronic myelogenous leukemia cell) cell lysates, A431, SK-Br-3, rat testis tissue, human thyroid gland tissue, human skin tissue, human breast cancer tissue, human pancreas tissue, mouse kidney tissue, mouse heart tissue.
Subcellular location: Cytoplasm. Plasma membrane. Nucleus.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
1:500-1:2000
1:50-1:200
1:50-1:200
Uniprot #: SwissProt: Q01844 Human | Q61545 Mouse
Entrez Gene: 289752 Rat
Alternative names: bK984G1.4 bK984G1.4 Ewing sarcoma breakpoint region 1 protein Ewing sarcoma breakpoint region 1 Ewing sarcoma breakpoint region 1 protein Ewings sarcoma EWS Fli1 type 1 oncogene EWS EWS oncogene EWS RNA binding protein 1 EWS_HUMAN EWSR 1 Ewsr1 EWSR1 protein RNA binding protein EWS RNA-binding protein EWS
Images
ET7109-94_1.jpg Fig1: Western blot analysis of EWSR1/EWS on K-562 (Human chronic myelogenous leukemia cell) cell lysate with Rabbit anti-EWSR1/EWS antibody (ET7109-94) at 1/2,000 dilution.

Lysates/proteins at 10 µg/Lane.
Exposure time: 10 seconds; ECL: K1801

Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: ET7109-94, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

Predicted band size: 68 kDa
Observed band size: 80 kDa
ET7109-94_2.jpg Fig2: ICC staining of EWSR1/EWS in A431 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7109-94, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET7109-94_3.jpg Fig3: ICC staining of EWSR1/EWS in SK-Br-3 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7109-94, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET7109-94_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-EWSR1/EWS antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-94, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7109-94_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using anti-EWSR1/EWS antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-94, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7109-94_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-EWSR1/EWS antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-94, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7109-94_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-EWSR1/EWS antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-94, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7109-94_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-EWSR1/EWS antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-94, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7109-94_9.jpg Fig9: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-EWSR1/EWS antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-94, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET7109-94_10.jpg Fig10: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-EWSR1/EWS antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-94, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.