Lactoferrin Recombinant Rabbit Monoclonal Antibody [JE49-60]
cat.: ET7109-95
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IF-Tissue, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE49-60 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 78 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Lactoferrin aa 26-221 / 710. |
| Positive control: | Human plasma lysates, human spleen tissue, human prostate cancer tissue. |
| Subcellular location: | Cytoplasm. Nucleus. Secreted. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue IF-Tissue IHC-P |
1:500-1:2,000 1:100 1:100 1:50-1:100 1:50-1:200 |
| Uniprot #: | SwissProt: P02788 Human |
| Alternative names: | GIG12 Growth inhibiting protein 12 HLF2 Kaliocin 1 Lactoferrin Lactoferroxin-C Lactotransferrin LF LTF Neutrophil lactoferrin Talalactoferrin TRFL_HUMAN |
Images
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Fig1:
Western blot analysis of Lactoferrin on human plasma lysate with Rabbit anti-Lactoferrin antibody (ET7109-95) at 1/2,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ET7109-95, 1/2,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 78 kDa Observed band size: 78 kDa |
|
Fig2: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Lactoferrin antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-95, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3: Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-Lactoferrin antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-95, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.