Lactoferrin Recombinant Rabbit Monoclonal Antibody [JE49-60]
cat.: ET7109-95
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IF-Tissue, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE49-60 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 78 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Lactoferrin aa 26-221 / 710. |
| Positive control: | Human placenta tissue lysates, human kidney tissue lysates, human spleen tissue, human prostate cancer tissue. |
| Subcellular location: | Cytoplasm. Nucleus. Secreted. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue IF-Tissue IHC-P |
1:500-1:2,000 1:100 1:100 1:50-1:100 1:50-1:200 |
| Uniprot #: | SwissProt: P02788 Human |
| Alternative names: | GIG12 Growth inhibiting protein 12 HLF2 Kaliocin 1 Lactoferrin Lactoferroxin-C Lactotransferrin LF LTF Neutrophil lactoferrin Talalactoferrin TRFL_HUMAN |
Images
|
Fig1:
Western blot analysis of Lactoferrin on human placenta tissue lysates with Rabbit anti-Lactoferrin antibody (ET7109-95) at 1/500 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 78 kDa Observed band size: 78 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7109-95) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig2: Western blot analysis of Lactoferrin on human kidney tissue lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. |
|
Fig3: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Lactoferrin antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-95, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4: Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-Lactoferrin antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-95, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.