ABAT / GABA-T Recombinant Rabbit Monoclonal Antibody [JE50-16]
cat.: ET7110-01
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | JE50-16 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 56 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human ABAT / GABA-T aa 1-124 / 500. |
| Positive control: | HepG2 cell lysate, Neuro-2a cell lysate, human liver tissue lysate, rat liver tissue lysate, human liver cancer tissue, human placenta tissue, mouse kidney tissue. |
| Subcellular location: | Mitochondrion. |
| Recommended Dilutions:
WB IP IHC-P |
1:500-1:2,000 1:50 1:50-1:200 |
| Uniprot #: | SwissProt: P80404 Human | P61922 Mouse | P50554 Rat |
| Alternative names: | (S) 3 amino 2 methylpropionate transaminase (S)-3-amino-2-methylpropionate transaminase 4 aminobutyrate aminotransferase 4 aminobutyrate aminotransferase, mitochondrial 4-aminobutyrate aminotransferase ABAT FLJ17813 FLJ30272 GABA aminotransferase GABA AT GABA T GABA transaminase GABA transferase GABA-AT GABA-T GABAT GABT_HUMAN Gamma amino N butyrate transaminase Gamma-amino-N-butyrate transaminase hCG1984265 L AIBAT L-AIBAT LAIBAT mitochondrial NPD009 |
Images
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Fig1:
Western blot analysis of ABAT / GABA-T on different lysates with Rabbit anti-ABAT / GABA-T antibody (ET7110-01) at 1/2,000 dilution. Lane 1: HepG2 cell lysate Lane 2: Neuro-2a cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 56 kDa Observed band size: 50 kDa Exposure time: Lane 1: 18 seconds; Lane 2: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7110-01) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of ABAT / GABA-T on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7110-01, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Human liver tissue lysate Lane 2: Rat liver tissue lysate |
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Fig3: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-ABAT / GABA-T antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-01, 1/200) for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-ABAT / GABA-T antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-01, 1/200) for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-ABAT / GABA-T antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-01, 1/200) for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.